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Characterization of a panel of somatic cell hybrids for subregional mapping along 11p and within band 11p13

Subdivision of the WAGR complex region

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Summary

The short arm of chromosome 11 carries genes involved in malformation syndromes, including the aniridia/genitourinary abnormalities/mental retardation (WAGR) syndrome and the Beckwith-Wiedemann syndrome, both of which are associated with an increased risk of childhood malignancy. Evidence comes from constitutional chromosomal aberrations and from losses of heterozygosity, limited to tumor cells, involving regions 11p13 and 11p15. In order to map the genes involved more precisely, we have fused a mouse cell line with cell lines from patients with constitutional deletions or translocations. Characterization of somatic cell hybrids with 11p-specific DNA markers has allowed us to subdivide the short arm into 11 subregions, 7 of which belong to band 11p13. We have thus defined the smallest region of overlap for the Wilms' tumor locus bracketed by the closest proximal and distal breakpoints in two of these hybrids. The region associated with the Beckwith-Wiedemann syndrome spans the region flanked by two 11p15.5 markers, HRAS1 and HBB. These hybrids also represent useful tools for mapping new markers to this region of the human genome.

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Authors and Affiliations

  1. Unité 73: Génétique et Pathologie Foetale, Institut National de la Santé et de la Recherche Médicale, Chateau de Longchamp, Bois de Boulogne, F-75016, Paris, France

    P. Couillin, M. Azoulay, I. Henry, N. Ravisé, M. C. Grisard, C. Jeanpierre, F. Barichard, J. J. Candelier & C. Junien

  2. Institut Pasteur Sc no 9 — Institut Pasteur, Institut National de la Santé et de la Recherche Médicale, Rue du Dr. Roux, F-75015, Paris, France

    P. Metezeau

  3. Department of Microbiology and Surgery, Medical Sciences Building, University of Toronto, M5S 1A8, Toronto, Canada

    W. Lewis

  4. Medical Research Council, Clinical and Population Cytogenetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK

    V. Van Heyningen

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  1. P. Couillin
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  2. M. Azoulay
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  3. I. Henry
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  4. N. Ravisé
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  5. M. C. Grisard
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  6. C. Jeanpierre
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  7. F. Barichard
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  8. P. Metezeau
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  9. J. J. Candelier
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  10. W. Lewis
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  11. V. Van Heyningen
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  12. C. Junien
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Couillin, P., Azoulay, M., Henry, I. et al. Characterization of a panel of somatic cell hybrids for subregional mapping along 11p and within band 11p13. Hum Genet 82, 171–178 (1989). https://doi.org/10.1007/BF00284053

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  • DOI: https://doi.org/10.1007/BF00284053

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