Summary
Conditioned culture medium from Daudi cells was used as a source of soluble H-Y antigen. Concentrated culture medium was labeled with 125I and then fractionated by gel filtration. Column fractions were assayed for the presence of H-Y antigen by urease-ELISA. H-Y antigen-containing fractions were then pooled and subjected to an improved immunoprecipitation protocol. Three predominant H-Y antigenic proteins were identified with estimated molecular weights of above 200,000, 50,000, and 20,000.
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Bradley, M.P., Heslop, B.F. A biochemical and immunological approach to the identification of H-Y antigentic proteins secreted from Daudi cells. Hum Genet 71, 117–121 (1985). https://doi.org/10.1007/BF00283365
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DOI: https://doi.org/10.1007/BF00283365