Summary
Since the last report numerous new DBP (Gc) variants have been observed; at present a total of 84 different mutants can be distinguished. Several of them have similar electrophoretic mobilities and/or isoelectric points by conventional isoelectric focusing (IEF). IEF in polyacrylamide gels in the presence of 3 M urea is a convenient and effecient method for the detection of hidden variation.
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Constans, J., Cleve, H., Dykes, D. et al. The polymorphism of the vitamin D-binding protein (Gc); Isoelectric focusing in 3 M urea as additional method for identification of genetic variants. Hum Genet 65, 176–180 (1983). https://doi.org/10.1007/BF00286658
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DOI: https://doi.org/10.1007/BF00286658