Summary
H-Y antigen was examined in XX-, XY-, and XO-mice using spleen, kidney, and liver cells of the animals for the absorption of the anti-H-Y antiserum produced in the rat. The cells of the XY- and XO-mice were found to be H-Y antigenpositive while the cells of the XX-mice were negative. As in Turner syndrome patients with 45,X, in the XO-female mice the H-Y antigen titre was reduced as compared to normal XY-male mice; intermediate values between those of normal male and female mice were obtained. These results clearly indicate that as in man, in the mouse the structural gene for H-Y antigen is not Y-linked but is located on an autosome. Furthermore, the concept of the regulation of the H-Y antigen gene expression in the human (Wolf et al. 1980a, b) by an X-linked repressor gene, escaping X-inactivation in the XX-female and an Y-linked inducer gene also seems to hold true in the mouse.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bennett D, Boyse EA, Mathieson BJ, Scheid M, Wachtel SS, Yanagisawa K, Cattanach BM (1977) Serological evidence for H-Y antigen in Sxr-XX sex-reversed phenotypic males. Nature 265:255–257
Boué J, Boué A (1973) Anomalies chromosomiques dans les avortements spontanés. In: Boué A, Thibault C (eds) Les accidents chromosomiques de la réproduction. INSERM, Paris, p 29
Cattanach BM (1962) XO mice. Genet Res 3:487–490
Cattanach BM (1974) Genetic disorders of sex determination in mice and other mammals. Birth Defects 4:129–141
Faggiano M, Ferraro M, Criscuolo T, Sinisi AA, de Capoa A (1980) Cytological evidence for the localisation of male-determining and H-Y genes on the short arm of Y-chromosome. Hum Genet 54: 323–326
Fellous M, Günther E, Kemler R, Wiels J, Berger R, Guenet JL, Jakob J, Jacob F (1978) Association of the H-Y male antigen with β2 in human lymphoid and differentiated mouse teratocarcinoma cell lines. J Exp Med 148:58–70
Ford CE (1969) Mosaics and chimaeras. Br Med Bull 25:104–109
German JL (1970) Abnormalities of human sex chromosomes. V. A unifying concept in relation to the gonadal dysgeneses. Clin Genet 1:15–27
Koo GC, Wachtel SS, Krupen-Brown K, Mittle LR, Breg WR, Geuel M, Rosenthal IM, Bargaonkar DS, Miller DA, Tantravahi R, Schreck RR, Erlanger BF, Miller OJ (1977) Mapping the locus of the H-Y gene of the human Y-chromosome. Science 198:940–942
Lyon MF (1961) Gene action in the X-chromosome of the mouse (Mus musculus L.). Nature 190:372–373
Lyon MF, Hawker SG (1973) Reproductive lifespan in irradiated and unirradiated chromosomally XO mice. Genet Res 21:185–194
Moreira-Filho CA, Otto PG, Frota-Pessoa O (1979) H-Y gene expression in apparent absence of the long arm of the Y-chromosome. Am J Med Genet 4:135–139
Morris T (1968) The XO and OY chromosome constitutions in mouse. Genet Res 12:125–137
Müller U, Zenzes MT, Wolf U, Engel W, Weniger J-R (1979) Appearance of H-W (H-Y) antigen in the gonads of oestradiol sexreversed male chicken embryos. Nature 280:142–144
Ohno S (1973) Ancient linkage group and frozen accidents. Nature 244:259–262
Ohno S (1979) Major sex determining genes. Springer, Berlin Heidelberg New York
Wachtel SS, Koo GC, Breg WR, Thaler HT, Dillard GM, Rosenthal IM, Dosik H, Gerald PS, Saenger P, New M, Lieber E, Miller OJ (1976) Serological detection of a Y-linked gene in XX males and XX true hermaphrodites. N Engl J Med 295:750–754
Wachtel SS, Koo GC, Ohno S (1977) H-Y antigen and male development. In: Troen P, Nankin HR (eds) The testis in normal and infertile man. Raven Press, New York, p 35
Wachtel SS, Bresler PA, Koide SS (1980) Does H-Y antigen induce the heterogametic ovary? Cell 20:859–864
Warburton D, Kline J, Susser M (1980) Monosomy X: A chromosomal anomaly associated with young maternal age. Lancet I:167–169
Welshons WJ, Russell LB (1959) The Y-chromosome as the bearer of male determining factors in the mouse. Proc Natl Acad Sci USA 45:560–566
Wolf U (1978) Zum Mechanismus der Gonadendifferenzierung. Bull Schweiz Akad Med Wiss 34:357–368
Wolf U, Fraccaro M, Mayerová A, Hecht T, Maraschio P, Hameister H (1980a) A gene controlling H-Y antigen on the X-chromosome. Tentative assignment by deletion mapping to Xp 223. Hum Genet 54:149–154
Wolf U, Fraccaro M, Mayerová A, Hecht T, Zuffardi O, Hameister H (1980b) Turner patients are H-Y positive. Hum Genet 54:315–318
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Engel, W., Klemme, B. & Ebrecht, A. Serological evidence for H-Y antigen in XO-female mice. Hum Genet 57, 68–70 (1981). https://doi.org/10.1007/BF00271170
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00271170