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Die blutgruppenassoziierten Elektrophoresetypen der alkalischen Serumphosphatase bei Gesunden und bei Patienten mit gastrointestinalen Erkrankungen

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Abstract

The alkaline serum phosphatase has been studied by starch gel electrophoresis in 746 healthy individuals, 62 patients with duodenal ulcer, 95 patients with stomach ulcer, and 65 patients with stomach cancer (Fig. 1, Table 1). The previously reported quantitative variations of the B-component of alkaline phosphatase, which is presumably identical with the intestinal component, has been confirmed. The variations of the B-component are associated with the ABO blood group system, the Lewis blood group system, and the ABH secretor status; they are also influenced by diet.

In a sample of 637 healthy, nonfasting persons the B-component was positively correlated to the O, B and Le(a-/b+) blood groups and to ABH secretion. The B-component was negatively associated with the A1 and Le(a+/b-) blood groups and the ABH non-secretor status (Table 2 and 3).

In a sample of 109 fasting healthy individuals the B-component was less frequent and also less intense if present. Nevertheless, the same correlations to the ABO and Lewis blood groups and the ABH secretor status are found as in the non-fasting control (Table 5 and 6).

In three persons the increase of the B-component of alkaline phosphatase in serum was followed after intake of a fatty meal (Table 7, Fig. 2 and 3).

In patients with duodenal ulcer, stomach ulcer and stomach cancer the B-component is less frequent and less intense than in the control group of fasting healthy individuals. This deviation is related to differences in the diet or to disturbances of the gastrointestinal functions in these patients. There was no clear indication of a connection between the B-component of alkaline phosphatase and the ABO blood groups association with these gastrointestinal diseases (Table 8, 9, and 10).

Zusammenfassung

Mit Hilfe der Stärkegelelektrophorese wurden die phänotypischen Variationen der alkalischen Serumphosphatase bei 746 Gesunden, 62 Patienten mit Ulcus duodeni, 95 Patienten mit Ulcus ventriculi und 65 Patienten mit Magencarcinom untersucht. Die Beziehungen der quantitativen Variationen der langsamer wandernden B-Komponente zum ABO-Blutgruppensystem, zum Lewis-Blutgruppensystem, zum ABH-Ausscheidersystem und ihre Abhängigkeit von der Nahrungszufuhr werden bestätigt.

Bei der Untersuchung von 637 nicht nüchternen Gesunden wurde eine positive Korrelation der B-Bande zu den Blutgruppen O, B und Le(a-/b+) und den Ausscheidern von ABH-Substanzen gefunden. Eine negative Korrelation der B-Bande fand sich zu den Blutgruppen A1, Le(a+/b-) und den Nichtausscheidern von ABH-Substanzen.

Bei einem Vergleichskollektiv von 109 nüchternen Gesunden wurde im Prinzip wieder die gleiche Korrelation gefunden. Die Häufigkeit und auch die Intensität der B-Bande war jedoch insgesamt geringer als bei den nicht nüchternen Gesunden.

Nach Nahrungsaufnahme kommt es zu einem Anstieg der Intensität der B-Komponente der alkalischen Phosphatase im Serum, wie in einem Diätversuch bei drei Personen gezeigt werden konnte.

Bei Patienten mit Ulcus duodeni, Ulcus ventriculi und Carcinoma ventriculi war sowohl die Häufigkeit als auch die Intensität der B-Bande geringer als bei Gesunden. Diese Abweichung wird auf eine Änderung der Ernährungsweise oder eine Störung im Bereich des Gastrointestinaltraktes zurückgeführt. Für einen Zusammenhang zwischen der B-Komponente der alkalischen Serumphosphatase und der bekannten ABO-Blutgruppenassoziation der drei untersuchten gastrointestinalen Erkrankungen ergab sich kein eindeutiger Hinweis.

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Literatur

  • Aird, I., H. H. Bentall, and J. A. F. Roberts: A relationship between cancer of stomach and the ABO blood groups. Brit. med. J. 1953/I, 799–801.

  • Aird, I., H. H. Bentall, J. A. Mehigan, and J. A. F. Roberts: The blood groups in relation to peptic ulceration and carcinoma of colon, rectum, breast, and bronchus. Brit. med. J. 1954/II, 315–321.

  • Arfors, K.-E., L. Beckman, and L.-G. Lundin: Genetic variations of human serum phosphatases. Acta genet. (Basel) 13, 89–94 (1963).

    Google Scholar 

  • Bamford, K. F., H. Harris, J. E. Luffman, E. B. Robson, and T. E. Cleghorn: Serum-alkaline-phosphatase and the ABO blood groups. Lancet 1965/I, 530–531.

  • Beckman, L.: Association between human serum alkaline phosphatases and blood groups. Acta genet. (Basel) 14, 286–297 (1964).

    Google Scholar 

  • —: Human alkaline phosphatases and the factors controlling their appearance in serum. Acta genet. (Basel) 16, 305–312 (1966).

    Google Scholar 

  • Blomstrand, R., and W. Werner: Alkaline phosphate activity in human thoracic duct lymph. Acta chir. scand. 129, 177–191 (1965).

    Google Scholar 

  • Boyer, S. H.: Alkaline phosphatase in human sera and placentae. Science 134, 1002–1004 (1961).

    Google Scholar 

  • —: Human organ alkaline phosphatase: Discrimination by several means including starch gel electrophoresis of antienzyme-enzyme supernatant fluids. Ann. N.Y. Acad. Sci. 103, 938–951 (1963).

    Google Scholar 

  • Chiandussi, L., S. F. Greene, and S. Sherlock: Serum alkaline phosphatase fractions in hepato-biliary and bone diseases. Clin. Sci. 22, 425–434 (1962).

    Google Scholar 

  • Clarke, C. A.: Blood groups and disease. Progr. med. Genet. 1, 81–119 (1961).

    Google Scholar 

  • Clarke, C. A. W. K. Cowan, J. W. Edwards, A. W. Howel Evans, R. B. McConnell, J. C. Woodrow, and P. M. Sheppard: The relationship of the ABO blood groups to duodenal and gastric ulceration. Brit. med. J. 1955/II, 643–646.

  • Cunningham, V. R., and J. G. Rimer: Isoenzymes of alkaline phosphatase of human serum. Biochem. J. 89, 50–51 (1963).

    Google Scholar 

  • Fishman, W. H., S. Green, and N. I. Inglis: Organ-specific behavior exhibited by rat intestine and liver alkaline phosphatase. Biochim. biophys. Acta (Amst.) 62, 363–375 (1962).

    Google Scholar 

  • —: L-Phenylalanine: an organ specific, stereospecific inhibitor of human intestinal alkaline phosphatase. Nature (Lond.) 198, 685–686 (1963).

    Google Scholar 

  • Gahne, B.: Genetic variation of phosphatase in cattle serum. Nature (Lond.) 199, 305–306 (1963).

    Google Scholar 

  • Gould, B. S.: Studies on the source of serum phosphatase. The nature of the increased serum phosphatase in rats after fat feeding. Arch. Biochem. 4, 175–181 (1944).

    Google Scholar 

  • Gutman, A. B.: Serum alkaline phosphatase activity in diseases of the skeletal and hepatobiliary systems. A consideration of the current status. Amer. J. Med. 27, 875–901 (1959).

    Google Scholar 

  • Hodson, A. W., A. L. Latner, and L. Raine: Isoenzymes of alkaline phosphatase. Clin. chim. Acta 7, 255–261 (1962).

    Google Scholar 

  • Hoppe, H. H.: Die Häufigkeitsverteilung der Blutgruppensysteme ABO, MN, Rh, P und K in Hamburg. Blut 3, 1–14 (1957).

    Google Scholar 

  • Kay, H. D.: Plasma phosphatase. I. Method of determination. Some properties of the enzyme. J. biol. Chem. 89, 235–247 (1930).

    Google Scholar 

  • Keiding, N. R.: The alkaline phosphatase fractions of human lymph. Clin. Sci. 26, 291–297 (1964).

    Google Scholar 

  • Kreisher, J. H., V. A. Close, and H. W. Fishman: Identification by means of L-Phenyl-alanine inhibition of intestinal alkaline phosphatase components separated by starch gel electrophoresis of serum. Clin. chim. Acta 11, 122–127 (1965).

    Google Scholar 

  • Krüpe, M.: Blutgruppenspezifische pflanzliche Eiweißkörper (Phytagglutinine). Stuttgart: Enke 1956.

    Google Scholar 

  • Langman, M. J. S., E. Leuthold, E. B. Robson, J. Harris, J. E. Luffman, and H. Harris: Influence of diet on the “intestinal” component of serum alkaline phosphatase in people of different ABO blood groups and secretor status. Nature (Lond.) 212, 41–43 (1966).

    Google Scholar 

  • Lawrence, S. H., and P. J. Melnick: Enzymatic activity related to human beta-lipoprotein: Histochemical, immuno-electrophoretic and quantitative studies. Proc. Soc. exp. Biol. (N.Y.) 107, 998–1001 (1961).

    Google Scholar 

  • —: A species comparison of serum proteins and enzymes by starch gel electrophoresis. Proc. Soc. exp. Biol. (N.Y.) 105, 572–575 (1960).

    Google Scholar 

  • Moss, D. W., R. H. Eaton, J. K. Smith, and L. G. Withby: Alteration in the electrophoretic mobility of alkaline phosphatases after treatment with Neuraminidase. Biochem. J. 98, 32c-33c (1966).

    Google Scholar 

  • Poulik, M. D.: Starch gel electrophoresis in a discontinuous system of buffers. Nature (Lond.) 180, 1477–1479 (1957).

    Google Scholar 

  • Price Evans, D. A.: Confirmation of association between ABO blood groups and salivary ABH secretor phenotypes and electrophoretic patterns of serum alkaline phosphatase. J. med. Genet. 2, 126–127 (1965).

    Google Scholar 

  • Rendel, J., and C. Stormont: Variants of ovine alkaline serum phosphatases and their association with the R-O blood groups (29056). Proc. Soc. exp. Biol. (N.Y.) 115, 853–856 (1964).

    Google Scholar 

  • Robinson, I. C., and I. E. Pierce: Differential action of neuraminidase on human serum alkaline phosphatase. Nature (Lond.) 204, 472–473 (1964).

    Google Scholar 

  • Roberts, J. A. F.: Blood groups and susceptibility to disease: A review. Brit. J. prev. soc. Med. 11, 107–125 (1957).

    Google Scholar 

  • Schlamowitz, M., and O. Bodansky: Tissue sources of human serum alkaline phosphatase, as determined by immunochemical procedures. J. biol. Chem. 234, 1433–1437 (1959).

    Google Scholar 

  • Shreffler, D. C.: Genetic studies of blood group-associated variations in a human serum alkaline phosphatase. Amer. J. hum. Genet. 17, 71–86 (1965).

    Google Scholar 

  • Smithies, O.: Zone electrophoresis in starch gels: Group variations in the serum proteins of normal human adults. Biochem. J. 61, 629–641 (1955).

    Google Scholar 

  • —: An improved procedure for starch-gel electrophoresis: Further variations in the serum proteins of normal individuals. Biochem. J. 71, 585–587 (1959).

    Google Scholar 

  • Watanabe, K., and W. H. Fishman: Application of stereospecific inhibitor L-Phenylalanine to the enzymorphology of intestinal alkaline phosphatase. J. Histochem. Cytochem. 12, 252–260 (1964).

    Google Scholar 

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Authors and Affiliations

  1. Institut für Humangenetik der Universität Marburg/Lahn, Marburg/Lahn, Deutschland

    M. Krüpe

  2. Staatliches Medizinal-Untersuchungsamt Fulda, Fulda, Deutschland

    G. G. Wendt

Authors
  1. K. Fichtner
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  2. H. Cleve
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  3. M. Krüpe
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  4. G. G. Wendt
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft, Bad Godesberg.

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Fichtner, K., Cleve, H., Krüpe, M. et al. Die blutgruppenassoziierten Elektrophoresetypen der alkalischen Serumphosphatase bei Gesunden und bei Patienten mit gastrointestinalen Erkrankungen. Hum Genet 4, 244–261 (1967). https://doi.org/10.1007/BF00292197

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  • DOI: https://doi.org/10.1007/BF00292197

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