Summary
Lymphocyte cultures of 120 normal persons evenly distributed in relation to sex and age groups were studied. A total of 3900 cells were examined, 3600 in 3-day cultures and 300 in cultivations maintained for 2 days. In both age groups (10–13; 62–96 years) men showed higher numbers of satellites per cell but less cells with D/G associations. Older subjects presented a lower number of cells with D satellites and acrocentric associations; the pattern of these associations, however, did not seem to vary with age. The mean number of associations per cell decreased significantly in 3-day as opposed to 2-day cultures.
Similar content being viewed by others
References
Aragatzuni, K. V.: Association of acrocentric chromosomes in 80–86 year-old persons (Russ.). Genetika 6, 79–85 (1967)
Bogomazov, E. A., Doroshenko, N. K.: Association of human acrocentric chromosomes and its dependence on the age of the individuals (Russ.). Genetika 7, 167–169 (1968)
Brink, J. M. van, Los, P. L., Nienhaus, A. J.: Satellite associations and the identification of the Y chromosome in man. Genetica 33, 45–51 (1962)
Cohen, M. M., Shaw, M. W.: The association of acrocentric chromosomes in 1000 normal male metaphase cells. Ann. hum. Genet. 31, 129–139 (1967)
Cooke, P.: Age-related variation in the number of secondary associations between acrocentric chromosomes in normal females and patients with Turner's syndrome. Humangenetik 17, 29–35 (1972)
Cristofalo, V. J.: Metabolic aspects of aging in diploid human cells. In: Aging in cell and tissue culture (eds. V. J. Cristofalo, E. Holečková), pp. 83–119. New York. Plenum Press 1970
Engmann, F. R.: Satellite-counts on mitotic chromosomes of monozygotic and dizygotic human twins. Lancet 1967 II, 1114–1115
Ferguson-Smith, M. A., Handmaker, S. D.: Observations on the satellited human chromosomes, Lancet 1961 I, 638–640
Ford, E. H. R., Woollam, D. H. M.: Significance of variation in satellite incidence in normal human mitotic chromosomes. Lancet 1967 II, 26–27
Frøland, A., Mikkelsen, M.: Studies on satellite associations in human cells. Hereditas (Lund) 52, 248 (1964)
Galperin, H.: Relative positions of homologous chromosomes or groups in male and female metaphase figures. Humangenetik 7, 265–274 (1969)
Goodman, R. M., Fechheimer, N. S., Miller, F., Miller, R., Zartman, D.: Chromosomal alterations in three age groups of human females. Amer. J. med. Sci. 258, 26–33 (1969)
Harnden, D. S.: The chromosomes. In: Recent advances in human genetics (ed. L. S. Penrose), pp. 19–29. Boston: Little, Brown 1961
Henderson, A. S., Warburton, D., Atwood, K. C.: Location of ribosomal DNA in the human chromosome complement. Proc. nat. Acad. Sci. (Wash.) 69, 3394–3398 (1972)
Mattevi, M. S., Salzano, F. M.: Senescence and human chromosome changes. Humangenetik 27, 1–8 (1975)
Nankin, H. R.: In vitro alteration of satellite association and nucleolar persistence in mitotic human lymphocytes. Cytogenetics 9, 42–51 (1970)
Ohno, S., Trujillo, J. M., Kaplan, W. D., Kinosita, R.: Nucleolus-organisers in the causation of chromosomal anomalies in man. Lancet 1961 II, 123–126
Polani, P. E., Briggs, J. H., Ford, C. E., Clarke, C. M., Berg, J. M.: A mongol girl with 46 chromosomes. Lancet 1960 II, 721–724
Prokofieva-Belgovskaya, A. A., Gindilis, V. M., Grinberg, K. N., Bogomasov, E. A., Podugolnikova, O. A., Isaeva, I. I., Radjabli, S. I., Cellarius, S. Ph., Veschneva, I. V.: Association of acrocentric chromosomes in relation to cell type and age of individuals. Exp. Cell Res. 49, 612–625 (1968)
Wulff, V. J., Quastler, H., Sherman, F. G.: An hypothesis concerning RNA metabolism and aging. Proc. nat. Acad. Sci. (Wash.) 48, 1373–1375 (1962)
Zang, K. D., Back, E.: Individual satellite association patterns. Lancet 1967 II, 1423
Zang, K. D., Back, E.: Quantitative studies on the arrangement of human metaphase chromosomes. I. Individual features in the association pattern of the acrocentric chromosomes of normal males and females. Cytogenetics 7, 455–470 (1968)
Zhdanova, N. S.: The acrocentric chromosome associations in human lymphocytes (Russ.). Tsitologiya 14, 1098–1105 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mattevi, M.S., Salzano, F.M. Effect of sex, age and cultivation time on number of satellites and acrocentric associations in man. Humangenetik 29, 265–270 (1975). https://doi.org/10.1007/BF00394187
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00394187