Summary
The mitochondrial forms of NADP-IDH, NADP-MDH, NAD-MDH and AAT can be demonstrated electrophoretically for the first time in post-implantation mouse embryos at the 11th to 12th day of development after vaginal plug. The onset of synthesis of these enzymes is parallel to the differentiation of mitochondria during development. Moreover, the mitochondrial form of the NADP-MDH was used to study the functioning of different alleles at one gene locus during embryonic development. In the heterozygous offspring of the cross CBA x SM/J an electrophoretic pattern was first seen 270 hrs after vaginal plug; the three heteromeric bands of the tetrameric enzyme become visible in a binomial distribution. 295 hrs after fertilization the expected 5-banded pattern of the heterozygote is completed. These findings support the assumption that the different parental alleles at the M-NADP-MDH gene locus start sythesizing synchronously. Similar results are already known for the S-NADP-IDH and S-NADP-MDH.
Zusammenfassung
Die mitochondrialen Formen der NADP-IDH, NADP-MDH, NAD-MDH und AAT können während der Embryogenese der Maus erstmals nach Implantation, und zwar am 11.-12. Entwicklungstag nach Vaginalpfropf, elektrophoretisch nachgewiesen werden. Der Synthesebeginn dieser Enzyme ist mit der Mitochondrienentwicklung während der Embryogenese in einen Zusammenhang zu bringen. Am Beispiel der mitochondrialen Form der NADP-MDH haben wir weiterhin das erstmalige Auftreten der Produkte verschiedener Allele eines Genlocus während der Embryonalentwicklung geprüft. Bei heterozygoten Nachkommen der Kreuzung CBA x SM/J tritt erstmals 270 Std nach Vaginalpfropf ein Elektrophoresemuster auf; die Heteromere des tetrameren Isoenzyms sind in binomialer Verteilung sichtbar. 295 Std nach Befruchtung ist das zu erwartende 5-Bandenmuster des Heterozygoten vollständig. Diese Befunde lassen die Annahme zu, daß die untersuchten Allele synchron ihre Syntheseaktivität aufnehmen. Entsprechende Ergebnisse sind von der S-NADP-IDH und S-NADP-MDH bereits bekannt.
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Supported by the Deutsche Forschungsgemeinschaft (SFB 46).
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Engel, W. Onset of synthesis of mitochondrial enzymes during mouse development. Humangenetik 20, 133–140 (1973). https://doi.org/10.1007/BF00284848
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DOI: https://doi.org/10.1007/BF00284848