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Early developmental changes of the lactate dehydrogenase isoenzyme pattern in mouse, rat, guinea-pig, Syrian hamster and rabbit

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Summary

The LDH-isoenzyme pattern was studied by microdisc electrophoresis in pre-implantation and early post-implantation embryos of the mouse, rat, guinea-pig and Syrian hamster. Prior to implantation only LDH1 (β subunits) is present. After implantation only LDH5 (α subunits) is demonstrable; additional isoenzymes appear subsequently. This indicates that in rodent species in general the total pre-implantation LDH activity is based on the maternally transmitted β subunits while the activation of the embryonal LDH genes starts only with implantation. Moreover, the gene for α-subunits is activated first and the gene for β subunits follows later on. As in rodents, LDH1 is also the only LDH isoenzyme present in the ovarian oocyte in the rabbit. However, in contrast to rodents, the embryonal LDH genes start functioning long before implantation in the rabbit: in the 86-hour-old rabbit embryo (middle blastocyst) isoenzymes formed of α and β subunits are already present.

Zusammenfassung

Bei Maus, Ratte, Meerschwein und Goldhamster wurde das LDH-Isoenzymmuster während der preimplantativen und frühen postimplantativen Entwicklung mit Hilfe der Mikro-Disk-Elektrophorese untersucht. Dabei fand sich vor der Implantation nur LDH1 (β-Untereinheiten), nach der Implantation zunächst nur LDH5 (α-Untereinheiten); weitere Isoenzyme kamen in Abhängigkeit vom Alter der Embryonen später hinzu. Daraus kann geschlossen werden, daß bei Rodentiern allgemein während der preimplantativen Entwicklung ausschließlich der aus β-Ketten bestehende mütterlich übertragen LDH-Vorrat vorhanden ist und die Aktivierung der embryonalen LDH-Gene erst nach der Implantation erfolgt. Dabei wird zuerst das Gen für α-Ketten und später das Gen für β-Ketten der LDH aktiviert. Im Gegensatz dazu werden beim Kaninchen offensichtlich die embryonalen LDH-Gene bereits längere Zeit vor der Implantation aktiviert: während in Oocyten aus dem Ovar wie bei Rodentiern ebenfalls nur β-Ketten nachweisbar sind, können bei 86 Std alten Embryonen (mittlere Blastocyste) mehrere LDH-Isoenzyme nachgewiesen werden, an deren Bildung α- und β-Untereinheiten beteiligt sind.

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Authors and Affiliations

  1. Institut für Humangenetik und Anthropologie der Universität, Freiburg i. Br.

    Wolfgang Engel

  2. Arbeitsgruppe Prof. G.H.M. Gottschewski am Max-Planck-Institut für Immunbiologie, Freiburg

    Ulrich Petzoldt

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  1. Wolfgang Engel
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  2. Ulrich Petzoldt
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Supported by the Deutsche Forschungsgemeinschaft

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Engel, W., Petzoldt, U. Early developmental changes of the lactate dehydrogenase isoenzyme pattern in mouse, rat, guinea-pig, Syrian hamster and rabbit. Humangenetik 20, 125–131 (1973). https://doi.org/10.1007/BF00284847

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  • DOI: https://doi.org/10.1007/BF00284847

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