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σB-dependent regulation ofgsiB in response to multiple stimuli inBacillus subtilis

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Abstract

The expression of thegsiB gene ofBacillus subtilis in response to a wide variety of stress conditions was analysed, and the results provide evidence thatgsiB is subject to a σB regulation. Primer extension experiments established identical start points forgsiB transcription during growth and after the induction by heat shock, salt or ethanol stress, and glucose limitation. The sequence upstream of the transcription start point shows great similarity to the sequences of σB promoters ofB. subtilis. σB was absolutely required for the increase ingsiB mRNA level and in the synthesis rate of GsiB in response to various stimuli. Measurements of the ATP pool indicated that changes in the level of ATP might be one of the signals that regulate the activity of σB inB. subtilis.

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Author notes

  1. Uwe Völker

    Present address: Department of Microbiology, The University of Texas, Health Science Center at San Antonio, 7703 Floyd Curl Drive, 78284-7758, San Antonio, Texas, USA

  2. Sabine Riethdorf

    Present address: Abteilung Histopathologie, Klinik für Geburtshilfe und Gynäkologie, Martinistrasse 52, 20246, Hamburg, Germany

Authors and Affiliations

  1. Institut für Mikrobiologie und Molekularbiologie, Universität Greifswald, Friedrich-Ludwig-Jahn-Strasse 15, 17487, Greifswald, Germany

    Björn Maul, Uwe Völker, Sabine Riethdorf, Susanne Engelmann & Michael Hecker

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  1. Björn Maul
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  2. Uwe Völker
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  3. Sabine Riethdorf
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  4. Susanne Engelmann
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  5. Michael Hecker
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Communicated by J. F. Lengeler

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Maul, B., Völker, U., Riethdorf, S. et al. σB-dependent regulation ofgsiB in response to multiple stimuli inBacillus subtilis . Molec. Gen. Genet. 248, 114–120 (1995). https://doi.org/10.1007/BF02456620

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  • DOI: https://doi.org/10.1007/BF02456620

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