Summary
Spontaneous mutants of Escherichia coli K12 displaying an increased level of the kanamycin resistance conferred by plasmid pGR71 were selected. Several mutants obtained in this way apparently carry large chromosomal deletions extending into galU and/or bglY (27 min). This positive selection of deletions allowed detection of a new locus located between galU and bglY. Deletions of this locus are responsible for increased resistance to kanamycin (Irk), decreased resistance to l-serine in minimal medium (Drs) and decreased resistance to chloramphenicol (Drc) when a cat gene is present in the bacteria.
References
Allen LN, Hanson RS (1985) Construction of broad-host-range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol. J Bacteriol 161:955–962
Bachmann BJ (1987) Linkage map of Escherichia coli K12, edition 7. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium cellular and molecular biology. American Society for Microbiology, Washington, pp 807–876
Cunningham PR, Clark DP (1986) The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol Gen Genet 205:487–493
Defez R, De Felice M (1981) Cryptic operon for β-glucoside metabolism in Escherichia coli K12: genetic evidence for a regulatory protein. Genetics 97:11–25
Fukasawa T, Jokura K, Kurahashi K (1962) A new enzymatic defect of galactose metabolism in Escherichia coli K12 mutants. Biochem Biophys Res Commun 7:121–125
Fukasawa T, Jokura K, Kurahashi K (1963) Mutations in Escherichia coli that affect uridine diphosphate glucose pyrophosphorylase activity and galactose fermentation. Biochim Biophys Acta 74:608–620
Goldfarb DS, Doi RH, Rodriguez RL (1981) Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis. Nature 293:309–311
Higgins CF, Dorman CJ, Stirling DA, Waddell L, Booth IR, May G, Bremer E (1988) A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli Cell 52:569–584
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Shaw WV (1975) Chloramphenicol acetyltransferase from chloramphenicol resistant bacteria. Methods Enzymol 43:737–755
Sundarajan TA, Rapin AMC, Kalckar HM (1962) Biochemical observations on E. coli mutants defective in uridine diphosphoglucose. Proc Natl Acad Sci USA 48:2187–2193
Uzan M, Danchin A (1976) A rapid test for the relA mutation in E. coli. Biochem Biophys Res Commun 69:751–758
Uzan M, Danchin A (1978) Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA mutants. Mol Gen Genet 165:21–30
Author information
Authors and Affiliations
Additional information
Communicated by J. Lengeler
Rights and permissions
About this article
Cite this article
Lejeune, P., Bertin, P., Walon, C. et al. A locus involved in kanamycin, chloramphenicol and L-serine resistance is located in the bglY-galU region of the Escherichia coli K12 chromosome. Molec Gen Genet 218, 361–363 (1989). https://doi.org/10.1007/BF00331292
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331292