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Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli

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Summary

The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.

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Authors and Affiliations

  1. Genetics Research Unit, Cell Biology Department, University of Auckland, Auckland, New Zealand

    Julie E. Croft, Donald R. Love & Peter L. Bergquist

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  1. Julie E. Croft
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  2. Donald R. Love
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  3. Peter L. Bergquist
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Communicated by M. Takanami

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Croft, J.E., Love, D.R. & Bergquist, P.L. Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli . Mol Gen Genet 210, 490–497 (1987). https://doi.org/10.1007/BF00327202

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  • DOI: https://doi.org/10.1007/BF00327202

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