Summary
A series of hybrid plasmids consisting of pC194 or pUB112 and B. subtilis DNA were constructed. In contrast to plasmid pC194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of PC194 derived plasmids, containing bacteriophage ϕ105 DNA were able to transform ϕ105 lysogenic but not nonylsogenic cells. From the results it is concluded that the presence of DNA/DNA homology between chromosomal DNA of the recipient cell and part of the hybrid plasmids used is a sufficient condition to endow monomeric plasmids with transforming activity.
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Communicated by E. Bautz
This work is part of the Doctoral Thesis to be submitted by A. Iglesias to the Freic Universität Berlin
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Bensi, G., Iglesias, A., Canosi, U. et al. Plasmid transformation in Bacillus subtilis . Mol Gen Genet 184, 400–404 (1981). https://doi.org/10.1007/BF00352512
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DOI: https://doi.org/10.1007/BF00352512