Skip to main content
SpringerLink
Log in

A Cis-acting regulator of enzyme tissue specificity in Drosophila is expressed at the RNA level

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Alcohol dehydrogenase (ADH) is expressed according to qualitatively different patterns in Drosophila grimshawi and two closely related species, D. orthofascia and D. formella. This regulatory difference is under control of a cis-acting genetic factor. The ADH of these species is very similar to ADH of D. melanogaster and a genomic ADH clone derived from the latter hybridizes specifically to ADH coding sequences of the other species. Using this hybridization probe, it is shown that the above cis-acting regulator acts before or shortly after transcription to control ADH expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abraham I, Doane WW (1978) Genetic regulation of tissue specific expression of amylase structural genes in Drosophila melanogaster. Proc Natl Acad Sci USA 75:4446–4450

    Google Scholar 

  • Alwine SC, Kemp DJ, Stark GR (1977) Method for detection of specific RNA's in agarose gels by transfer in diazobenzyloxymethyl paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74:5350–5354

    Google Scholar 

  • Anderson CW, Lewis JB, Atkins JF, Gesteland RF (1974) Cell free synthesis of adenovirus-2 proteins programmed by fractionated mRNA: A comparison of polypeptide products and mRNA lengths. Proc Natl Acad Sci USA 71:2756–2760

    Google Scholar 

  • Bailey JM, Davidson N (1976) Methyl mercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem 70:75–85

    Google Scholar 

  • Bonner WM, Laskey RA (1974) A film detection method for tritiumlabeled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83–88

    Google Scholar 

  • Boubelik M, Lengerova A, Bailey DW, Matousek V (1975) A model for genetic analysis of programmed gene expression as reflected in development of membrane antigens. Dev Biol 47:206–214

    Google Scholar 

  • Carson HL, Kaneshiro KY (1976) Drosophila of Hawaii: Systematics and ecological genetics. Annu Rev Ecol Syst 7:311–345

    Google Scholar 

  • Chovnick A, Gelbart W, McCarron M, Osmond B, Candido EPM, Baillie DL (1976) Organization of the rosy locus in Drosophila melanogaster: Evidence for a control element adjacent to the xanthine dehydrogenase structural clement. Genetics 84:232–255

    Google Scholar 

  • Clewell DB, Helinski DR (1969) Supercoiled circular DNA-protein complex in E. coli: Purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1163

    Google Scholar 

  • Daniel WL (1976) Genetic control of heat sensitivity and activity level of murine arylsulfatase B. Biochem Genet 14:1003–1018

    Google Scholar 

  • Dickinson WJ (1975) A genetic locus affecting the developmental expression of an enzyme in D. melanogaster. Dev Biol 47:206–214

    Google Scholar 

  • Dickinson WJ (1978) Genetic control of enzyme expression in Drosophila: A locus influencing tissue specificity of aldehyde oxidase. J Exp Zool 206:333–341

    Google Scholar 

  • Dickinson WJ (1980a) Tissue specificity of enzyme expression regulated by diffusible factors: Evidence from Drosophila hybrids. Science 207:995–997

    Google Scholar 

  • Dickinson WJ (1980b) Complex cis-acting regulatory genes demonstrated in Drosophila hybrids. Dev Gen 1:229–240

    Google Scholar 

  • Dickinson WJ (1980c) Evolution of patterns of gene expression in Hawaiian picture winged Drosophila. J Mol Evol 16:73–94

    Google Scholar 

  • Dickinson WJ, Carson HL (1979) Regulation of the tissue specificity of an enzyme by a cis-acting genetic element: Evidence from Drosophila hybrids. Proc Natl Acad Sci USA 76:4559–4562

    Google Scholar 

  • Freeling M, Cheng DSK (1978) Radiation induced alcohol dehydrogenase mutants in maize following allyl alcohol selection of pollen. Genet Res 31:107–129

    Google Scholar 

  • Gelbart WM, McCarron M, Pandey J, Chovnick A (1974) Genetic limits of the xanthine dehydrogenase structural element within the rosy locus in Drosophila melanogaster. Genetics 78:869–886

    Google Scholar 

  • Goldberg DA (1980) Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci USA 77:5794–5798

    Google Scholar 

  • Goldberg ML, Lifton RP, Stark GR, Williams JG (1979) Isolation of specific RNA's using DNA covalently linked to diazobenzyloxymethyl cellulose or paper. In: Wu R (ed) Methods in Enzymology, vol 68. Academic Press, New York, p 206–220

    Google Scholar 

  • Laemmli UK, Favre M (1973) Maturation of the head of bacteriophage T4: DNA packaging events. J Mol Biol 80:575–599

    Google Scholar 

  • Lehrach H, Fiamond D, Wozney JM, Boedtker H (1977) RNA molecular weight determination by gel electrophoresis under denaturing conditions: A critical reexamination. Biochemistry 16:4743–4751

    Google Scholar 

  • O'Donnell J, Mandel H, Krause M, Sofer W (1977) Genetic and cytogenetic analysis of the ADH region in Drosophila melanogaster Genetics 86:553–566

    Google Scholar 

  • Paigen K (1961) The genetic control of enzyme activity during differentiation. Proc Natl Acad Sci USA 47:1641–1649

    Google Scholar 

  • Paigen K (1963) The genetic control of enzyme realization during differentiation. In: Fishbein M (ed) 2nd Int'l. Conference on Congenital Malformation. Int Med Congr NY, pp 181–190

  • Paigen K (1979) Genetic factors in developmental regulation. In: Scandalios JG (ed) Physiological genetics. Academic Press, New York

    Google Scholar 

  • Paigen K, Meisler M, Felton J, Chapman V (1976) Genetic determination of the β-galasctosidase developmental program in mouse liver. Cell 9:533–539

    Google Scholar 

  • Pelham HRB, Jackson RJ (1976) An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem 67:247–256

    Google Scholar 

  • Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling DNA to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:239–251

    Google Scholar 

  • Schwartz D (1962) Genetic studies on mutant enzymes in maize: Control of gene action in synthesis of pH 7.5 esterase. Genetics 47:1609–1615

    Google Scholar 

  • Sofer W, Ursprung H (1968) Drosophila alcohol dehydrogenase: Purification and partial characterization. J Biol Chem 243:3110–3115

    Google Scholar 

  • Spradling AC, Mahowald AP (1980) Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci USA 77:1096–1100

    Google Scholar 

  • Thatcher DR (1977) Enzyme instability and proteolysis during purification of an alcohol dehydrogenase from Drosophila melanogaster. Biochem J 87:317–323

    Google Scholar 

  • Thomas PS (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77:5201–5205

    Google Scholar 

  • Thompson JN, Ashburner M, Woodruff RC (1977) Presumptive control mutation for alcohol dehydrogenase in Drosophila melanogaster. Nature 270:363

    Google Scholar 

  • Wahl GM, Stern M, Stark GP (1979) Efficient transfer of large DNA fragments from agarose gels to DBM paper and rapid hybridization using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687

    Google Scholar 

  • Wheeler MR, Clayton FE (1965) A new Drosophila culture technique. Dros Inf Serv 40:98

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, University of Utah, 84112, Salt Lake City, Utah, USA

    Leonard Rabinow & W. J. Dickinson

Authors
  1. Leonard Rabinow
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. J. Dickinson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by M.M. Green

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rabinow, L., Dickinson, W.J. A Cis-acting regulator of enzyme tissue specificity in Drosophila is expressed at the RNA level. Molec. Gen. Genet. 183, 264–269 (1981). https://doi.org/10.1007/BF00270627

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270627

Keywords

Search

Navigation