Summary
Saturation of a specific region of a chromosome with conditional lethal mutations becomes increasingly difficult as the genome size of the organism increases. We show that mutagenesis of cloned genes followed by their re-integration into a non-mutagenized organism is a pracfical way to circumvent this difficulty.
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Völker, T.A., Showe, M.K. Induction of mutations in specific genes of bacteriophage T4 using cloned restriction fragments and marker rescue. Molec. Gen. Genet. 177, 447–452 (1980). https://doi.org/10.1007/BF00271483
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DOI: https://doi.org/10.1007/BF00271483