Summary
Multiplication of TMV-strains vulgare (light-green/dark-green mosaic symptoms) and flavum (severe yellow/green mosaic) had different effects on the ribosomal RNA of tobacco leaf chloroplasts. Vulgare inhibited chloroplast ribosomal RNA synthesis while having no effect on cytoplasmic ribosomal RNA synthesis (Fig. 2). Flavum inhibited chloroplast ribosomal RNA synthesis more severely than vulgare, and caused an earlier degradation of chloroplast ribosomal RNA than in control or vulgare-infected leaves (Fig. 1). Flavum also inhibited cytoplasmic ribosomal RNA synthesis. A connection between these differing effects on chloroplast ribosomal RNA metabolism and severity of visible symptoms is suggested, and discussed in relation to a possible influence on symptoms of denatured virus coat protein.
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Abbreviations
- TMV:
-
Tobacco Mosaic Virus
- RNA:
-
Ribonucleic acid
- DNA:
-
Deoxyribonucleic acid
- m:
-
millions (in molecular weight values)
References
Bishop, D. H. L., Claybrook, J. R., Spiegelman, S.: Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J. molec. Biol. 26, 373–387 (1967).
Comar, C. L., Zscheile, F. P.: Analysis of plant extracts for chlorophylls a and b by a photoelectric spectrophotometric method. Plant Physiol. 17, 198–209 (1942).
Fraser, R. S. S.: The synthesis and properties of ribonucleic acid in dividing plant cells. Ph. D. Thesis, University of Edinburgh (1968).
Hastings, J. R. B., Kirby, K. S.: The nucleic acids of Drosophila melanogaster. Biochem. J. 100, 532–539 (1966).
Hirai, A., Wildman, S. G.: Effect of TMV multiplication on RNA and protein synthesis in tobacco chloroplasts. Virology 38, 73–82 (1969).
Ingle, J.: Synthesis and stability of chloroplast ribosomal RNAs. Plant Physiol. 43, 1448–1454 (1968).
Jockusch, H.: Two mutants of tobacco mosaic virus temperature-sensitive in two different functions. Virology 35, 94–101 (1968).
—, Jockusch, B.: Early cell death caused by TMV-mutants with defective coat proteins. Molec. Gen. Genetics 102, 204–209 (1968).
Kirby, K. S.: Isolation and characterisation of ribosomal ribonucleic acid. Biochem. J. 96, 266–269 (1965).
Kirk, J. T. O., Tilney-Basset, R. A. E., in: The Plastids. London: W. H. Freeman 1967.
Loening, U. E.: The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 102, 251–257 (1967).
—: The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J. 113, 131–138 (1969).
—, Ingle, J.: Diversity of RNA components in green plant tissues. Nature (Lond.) 215, 363–367 (1967).
Parish, J. H., Kirby, K. S.: Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim. biophys. Acta (Amst.) 129, 554–562 (1966).
Peterson, P. D., McKinney, H. H.: The influence of four mosaic diseases on the plastid pigments and chlorophyllase in tobacco leaves. Phytopathol. 28, 329–342 (1938).
Spencer, D., Whitfeld, P. R.: The nature of the ribonucleic acid of isolated chloroplasts. Arch. Biochem. 117, 337–346 (1966).
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Communicated by H. G. Wittmann
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Fraser, R.S.S. Effects of two TMV strains on the synthesis and stability of chloroplast ribosomal RNA in tobacco leaves. Molec. Gen. Genetics 106, 73–79 (1969). https://doi.org/10.1007/BF00332822
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DOI: https://doi.org/10.1007/BF00332822