Skip to main content
SpringerLink
Log in

Studies on the positive regulatory gene, GAL4, in regulation of galactose catabolic enzymes in Saccharomyces cerevisiae

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Studies were undertaken to elucidate the role of the GAL4 locus in regulating the galactose catabolic enzymes in Saccharomyces cerevisiae. The GAL4 locus has been proposed to have a regulatory function since GAL4 mutants are pleiotropic, lacking epimerase, galactokinase, transferase and galactose permease activities. This pleiotropic effect could be explained if the GAL4 gene codes for a peptide required for above enzyme activities. To study if the GAL4 gene codes for a structural component of the above enzymes, a temperature sensitive mutation mapping close to or within the GAL4 gene (gal4-1ts) was isolated. The results suggest that the GAL4 locus does not code for a polypeptide common to the transferase, epimerase, galactokinase, and permease enzymes since these enzymes extracted from the gal4-1ts mutant did not differ qualitatively in thermolability and temperature optima from those of the wild-type. It was found that in the gal4-1ts mutant the synthesis of the epimerase is temperature sensitive and that the GAL4 gene product does not have any effect on the in vivo stability of epimerase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Burkl, G., Castroph, H., Schweizer, E.: Mapping of a complex gene locus coding for part of the Saccharomyces cerevisiae fatty acid synthetase multienzyme complex. Mol. gen. Genet. 119, 315–322 (1972)

    CAS  PubMed  Google Scholar 

  • Douglas, H. C., Condi, F.: The genetic control of galactose utilization in Saccharomyes. J. Bact. 68, 662–670 (1954)

    CAS  PubMed  Google Scholar 

  • Douglas, H. C., Hawthorne, D. C.: Enzymatic expression and genetic linkage of genes controlling galactose utilization in Saccharomyces. Genetics 49, 837–844 (1964)

    CAS  PubMed  PubMed Central  Google Scholar 

  • Douglas, H. C., Hawthorne, D. C.: Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast. Genetics 54, 911–916 (1966)

    CAS  PubMed  PubMed Central  Google Scholar 

  • Douglas, H. C., Hawthorne, D. C.: Uninducible mutants in the gal i locus of Saccharomyces cerevisiae. J. Bact. 109, 1139–1143 (1972)

    CAS  PubMed  Google Scholar 

  • Gielow, L., Largen, M., Englesberg, E.: Initiator constitutive mutants of the L-arabinose operon (OIBAD) of Escherichia coli B/r. Genetics 69, 289–302 (1971)

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hawthorne, D. C., Friis, J.: Osmotic-remedial mutants. A new classification for nutritional mutants in yeast. Genetics 50, 829–839 (1970)

    Google Scholar 

  • Jacob, F., Monod, J.: Genetic regulatory mechanisms in the induced synthesis of proteins. J. molec. Biol. 3, 318–356 (1961)

    Article  CAS  Google Scholar 

  • Khan, N. A., Eaton, N. R.: Genetic control of maltase formation in yeast. I. Strains producing high and low basal levels of enzyme. Molec. gen. Genet. 112, 317–322 (1971)

    Article  CAS  Google Scholar 

  • Marzluf, G. A., Metzenberg, R. L.: Positive control the cys-3 locus in regulation of sulfur metabolism in Neurospora. J. molec. Biol. 33, 423–437 (1968)

    Article  CAS  Google Scholar 

  • Maxwell, E. S., Kurahashi, K., Kalcar, H. M.: Enzymes of the Leloir pathway. In: Methods in enzymology (S. P. Colowick and N. O. Kaplan, eds.), vol. V, p. 174–189. New York: Academic Press 1962

    Google Scholar 

  • Miller, J. H., Ippen, K., Scaife, J. G., Beckwith, J. R.: The promotor-operator region of the lac operon of Escherichia coli. J. molec. Biol. 38, 413–420 (1968)

    Article  CAS  Google Scholar 

  • Mortimer, R. K., Hawthorne, D. C.: Genetic mapping in Saccharomyces. Genetics 53 165–173 (1966)

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schatz, G., Groot, G. S. P., Mason, T., Rouslin, W., Wharton, D. C., Saltzgaber, J.: Biogenesis of mitochondrial inner embranes in Baker's yeast. Fed. Proc. 31, 21–29 (1972)

    CAS  PubMed  Google Scholar 

  • Sorger, G. J.: Nitrate reductase electron transport systems in mutant and in wild-type strains of Neurospora. Biochim. biophys. Acta (Amst.) 118, 484–494 (1966)

    Article  CAS  Google Scholar 

  • Valone, J. A., Jr., Case, M. E., Giles, N. H.: Constitutive mutants in a regulatory gene exerting positive control of quinic acid catabolism in Neurospora crassa. Proc. nat. Acad. Sci. (Wash.) 68, 1555–1559 (1971)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts

    Amar J. S. Klar & Harlyn O. Halvorson

Authors
  1. Amar J. S. Klar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Harlyn O. Halvorson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Maas

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klar, A.J.S., Halvorson, H.O. Studies on the positive regulatory gene, GAL4, in regulation of galactose catabolic enzymes in Saccharomyces cerevisiae . Molec. Gen. Genet. 135, 203–212 (1974). https://doi.org/10.1007/BF00268616

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268616

Keywords

Search

Navigation