Summary
E. coli x S.typhimurium partially diploid hybrids were constructed to investigate the possibility of genetic complementation between the SA and the SB restriction and modification systems of S. typhimurium and the K and B systems of E. coli.
An hsdR -K mutation was complemented in a stable hybrid in which the hsd +SA -hsd +SB -serB + portion of the S. typhimurium chromosome was integrated at a non-homologous locus. By isolating hsd - mutants in that hybrid, it was shown that complementation occured between K and SB, but not between K and SA.
Similarly, in a set of F-prime merodiploids bearing the SA, SB and B systems, complementation was observed between B and SB, but not between B and SA.
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Van Pel, A., Colson, C. DNA restriction and modification systems in Salmonella . Molec. Gen. Genet. 135, 51–60 (1974). https://doi.org/10.1007/BF00433901
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DOI: https://doi.org/10.1007/BF00433901