Summary
Two sets of plaque-forming λ transducing phages have been isolated which carry parts of the tryptophan (trp) and lactose (lac) operons. The λptrp-lac set of phages carry the trp and lac operons in the same orientation connected by deletions which enter the lac regulatory region from the i side. These deletions start at various sites in or near the trp operon and end either late in the lac i gene, within the promoter, between the promoter and the operator, within the operator, between the operator and the z gene, or very early in the z gene. Starting with one particular trp-lac fusion strain, a series of transducing phages were isolated which contain varying portions of the trp operon extending from the trp A gene towards the trp operator. The other set of phages, which are designated λptrp/lac, carry trp and lac in opposing orientations. These λptrp/lac phages contain deletions which remove all of the lac structural genes and end between the operator and the z gene.
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Arditti, R., Grodzicker, T., Beckwith, J.: Cyclic adenosine monophosphate-independent mutants of the lactose operon of Escherichia coli. J. Bact. 114, 652–655 (1973)
Beckwith, J., Grodzicker, T., Arditti, R.: Evidence for two sites in the lac promoter region. J. molec. Biol. 69, 155–160 (1972)
Blundell, M., Craig, E., Kennell, D.: Decay rates of different mRNA in E. coli and models of decay. Nature (Lond.) New Biol. 238, 46–49 (1972)
Davidson, M., Szybalski, W.: Physical and chemical characteristics of lambda DNA. The bacteriophage lambda (ed. A. Hershey), p. 45–82. New York: Cold Spring Harbor Laboratory (1971)
Deeb, S., Okamoto, K., Hall, B.: Isolation and characterization of non-defective transducing elements of bacteriophage ϕ80. Virology 31, 289–295 (1967)
Fiandt, M., Hradecna, Z., Lozeron, H. A., Szybalski, W.: Electron micrographic mapping of deletions, insertions, inversions, and homologies in the DNAs of coliphages lambda and phi 80. The bacteriophage lambda (ed. A. Hershey), p. 329–354. New York: Cold Spring Harbor Laboratory 1971
Franklin, N.: The N operon of lambda: Extent and regulation as observed in fusions to the tryptophan operon of Escherichia coli. The bacteriophage lambda (ed. A. Hershey), p. 621–638. New York: Cold Spring Harbor Laboratory 1971
Gilbert, W., Müller-Hill, B.: The lac operator is DNA. Proc. nat. Acad. Sci. (Wash.) 58, 2415–2421 (1967)
Gottesman, S., Beckwith, J. R.: Direct transposition of the arabinose operon: A technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene. J. molec. Biol. 44, 117–127 (1969)
Imamoto, F., Yanofsky, C.: Transcription of the tryptophan operon in polarity mutants of Escherichia coli: I. Characterization of the tryptophan messenger RNA of polar mutants. J. molec. Biol. 28, 1–23 (1967)
Ippen, K., Shapiro, J. A., Beckwith, J. R.: Transposition of the lac region of the Escherichia coli chromosome: Isolation of lac transducing bacteriophages. J. Bact. 108, 5–9 (1971)
Jacob, F., Monod, J.: Genetic regulatory mechanisms in the synthesis of proteins. J. molec. Biol. 3, 318–356 (1961)
Jacob, F., Perrin, D., Sanchez, C., Monod, J.: L'operon: Groupe de genes a expression coordonnee par un operateur. C. R. Acad. Sci. (Paris) 250, 1727–1729 (1960)
Jacob, F., Ullman, A., Monod, J.: Le promoteur, element genetique necessaire a l'expression d'un operon. C. R. Acad. Sci. (Paris) 258, 3125–3128 (1964)
Konrad, E., Kirschbaum, J., Austin, S.: Isolation and characterization of ϕ80 transducing bacteriophage for a ribonucleic acid polymerase gene. J. Bact. 116, 511–516 (1973)
Malamy, M. H., Fiandt, M., Szybalski, W.: Electron microscopy of polar insertions in the lac operon of Escherichia coli. Molec. gen. Genet. 119, 207–222 (1972)
Miller, J. H.: Transcription starts and stops in the lac operon. The lactose operon (ed. J. Beckwith and D. Zipser), p. 173–188. New York: Cold Spring Harbor Laboratory 1970
Miller, J. H., Ippen, K., Scaife, J. G., Beckwith, J. R.: The promoter-operator region of the lac operon of Escherichia coli. J. molec. Biol. 38, 413–420 (1968)
Miller, J. H., Reznikoff, W. S., Silverstone, A. E., Ippen, K., Signer, E. R., Beckwith, J. R.: Fusions of the lac and trp regions of the Escherichia coli chromosome. J. Bact. 104, 1273–1279 (1970)
Mitchell, D., Reznikoff, W. S., Beckwith, J. R.: Genetic fusions defining trp and lac operon regulatory elements. Submitted to J. molec. Biol. (1974)
Newton, W. A., Snell, E. E.: Catalytic properties of tryptophanase, a multifunctional pyridoxal phosphate enzyme. Proc. nat. Acad. Sci. (Wash.) 51, 382–389 (1964)
Pardee, A. B., Jacob, F., Monod, J.: The genetic control and cytoplasmic expression of inducibility in the synthesis of β-galactosidase of E. coli. J. molec. Biol. 1, 165–178 (1959)
Parkinson, J. S., Huskey, R. S.: Deletion mutants of bacteriophage lambda: I. Isolation and initial characterization. J. molec. Biol. 56, 369–384 (1971)
Platt, T., Weber, K., Ganem, D., Miller, J. H.: Translation restarts: AUG reinitiation of a lac repressor fragment. Proc. nat. Acad. Sci. (Wash.) 69, 897–901 (1972)
Press, R., Glansdorff, N., Miner, P., DeVries, J., Kadner, R., Maas, W. K.: Isolation of transducing particles of ϕ80 bacteriophage that carry different regions of the Escherichia coli genome. Proc. nat. Acad. Sci. (Wash.) 68, 795–798 (1971)
Reznikoff, W. S.: The operon revisited. Ann. Rev. Genet. 6, 133–156 (1972)
Reznikoff, W. S., Beckwith, J. R.: Genetic evidence that the operator locus is distinct from the z gene in the lac operon of Escherichia coli. J. molec. Biol. 43, 215–218 (1969)
Reznikoff, W. S., Miller, J. H., Scaife, J. G., Beckwith, J. R.: A mechanism for repressor action. J. molec. Biol. 43, 201–213 (1969)
Reznikoff, S. W., Thornton, K. P.: Isolating tryptophan regulatory mutants in Escherichia coli by using a trp-lac fusion strain. J. Bact. 109, 526–532 (1972)
Scaife, J., Beckwith, J. R.: Mutational alteration of the maximal level of lac operon expression. Cold Spr. Harb. Symp. quant. Biol. 34, 101–111 (1966)
Schrenk, W. J.: Genetische vorarbeiten zur isolation des trp-repressors in Escherichia coli. Ph. D. Thesis, Universität zu Köln (1972)
Shimada, K., Weisberg, R. A., Gottesman, M. E.: Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J. molec. Biol., in press (1973)
Signer, E. R., Beckwith, J. R.: Transposition of the lac region of E. coli. III. The mechanism of attachment of coliphage ϕ80 to the bacterial chromosome. J. molec. Biol. 22, 33–51 (1966)
Smith, O. H., Yanofsky, C.: Enzymes involved in the biosynthesis of tryptophan. III. Tryptophan synthetase. Methods in enzymology, vol. 5 (ed. S. Colowick and N. Kaplan), p. 801–806. New York: Academic Press 1963
Tomita, T.: On the transcription of the ϕ80 phage genome. Molec. gen. Genet. 103, 313–325 (1969)
Voll, M. J.: Derivation of an F-merogenote and a ϕ80 frequency transducing phage carrying the histidine operon of Salmonella. J. Bact. 109, 741–780 (1972)
Wilcox, G., Singer, J., Heffernan, L.: Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-arabinose operon of Escherichia coli B/r. J. Bact. 108, 1–4 (1971)
Yanofsky, C., Horn, V., Bonner, M., Stasiowsky, S.: Polarity of enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli. Genetics 69, 409–433 (1971)
Zubay, G., Morse, D. E., Schrenk, W. J., Miller, J. H.: Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 69, 1100–1103 (1972)
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Communicated by W. Maas
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Barnes, W.M., Siegel, R.B. & Reznikoff, W.S. The construction of λ transducing phages containing deletions defining regulatory elements of the lac and trp operons in E. coli . Molec. Gen. Genet. 129, 201–215 (1974). https://doi.org/10.1007/BF00267913
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DOI: https://doi.org/10.1007/BF00267913