Summary
In vivo transcription of B. subtilis phage SPP1 was studied by hybridization between RNA, pulse labelled with 3H-uridine at various times during phage development, and separated complementary strands (H-and L-strand) of SPP1 DNA. 3H RNA isolated from cultures of B. subtilis 168-2 early in SPP1 infection hybridizes exclusively with the H-strand. For a short period of time about 20 min after infection RNA is found which also hybridizes with the L-strand. Very late in infection RNA is again transcribed preferentially from the H-strand.
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Communicated by E. Bautz
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Esche, H., Spatz, H.C. Asymmetric transcription of SPP1 in vivo . Molec. Gen. Genet. 124, 57–63 (1973). https://doi.org/10.1007/BF00267164
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DOI: https://doi.org/10.1007/BF00267164