Summary
“Functional” mutants of phage SP50 are described whose growth, in contrast to wild type SP50, is restricted on B. subtilis strain 168. On the basis of complementation tests and genetic crosses, these mutations could be grouped into three genes. Three types of mutants of strain 168, which permitted the growth of any one of the three classes of phage mutants were isolated.
In transfection of 168 with SP50 wild type DNA the number of infective centers varies with the third to fourth power of the DNA concentration. Infection of competent 168 cells with an intact functional-mutant phage concomitant with transfection with wild type DNA greatly enhances the specific activity of such DNA and leads to a linear dependence between the number of infected centers and DNA concentration.
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Communicated by G. Bertani
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Rottländer, E., Trautner, T.A. Genetic and transfection studies with B. subtilis phage SP 50 . Molec. Gen. Genetics 108, 47–60 (1970). https://doi.org/10.1007/BF00343184
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DOI: https://doi.org/10.1007/BF00343184