Skip to main content
SpringerLink
Log in

Alkylation induced gene conversion in yeast: Use in fine structure mapping

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Methyl-methanesulfonate (MMS) causes gene conversions in heteroallelic diploids of Saccharomyces cerevisiae. The frequency of production of prototrophic convertants is linearly proportional to the square of the time of MMS treatment, and the regression of prototrophs on dose varies depending upon the particular pair of alleles present in the diploid. The regressions show an additivity relationship, in that when a triad of heteroallelic diploids of the type m1/m2, m2/m3, and m1/m3 is considered, two of the regressions add up approximately to the third. MMS can, therefore, be used in fine structure mitotic mapping of genes. Good agreement was found both in relative order and spacing of alleles at the histidine 1 locus of yeast when the fine structure map based on the X-ray mapping method was compared with that based on MMS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Boyce, R. P., Farley, J. W.: Methyl-methanesulfonate-induced single strand breaks and joining of broken strands in covalent circular λ-DNA in superinfected lysogens. Biophys. J. Abstr. 8, 50 (1968).

    Google Scholar 

  • Brookes, P., Lawley, P. D.: Reaction of some mutagenic and carcinogenic compounds with nucleic acids. J. cell. comp. Physiol. 64, Suppl. 1, 111–128 (1964).

    Google Scholar 

  • Eisenstark, A., Eisenstark, R., Dillewijn, J. van, Rörsch, A.: Radiation-sensitive (uvr) and recombinationless (rec) mutants of Salmonella typhimurium. Bact. Proc., 36 (1968).

  • Freifelder, D.: Mechanism of inactivation of coli-phage T7 by X-rays. Proc. nat. Acad. Sci. (Wash.) 54, 128–134 (1965).

    Google Scholar 

  • —: DNA strand breakage by X-irradiation. Radiat. Res. 29, 329–338 (1966).

    Google Scholar 

  • —: Rate of production of single-strand breaks in DNA by X-irradiation in situ. J. molec. Biol. 35, 303–309 (1968).

    Google Scholar 

  • Friedberg, E. C., Goldthwait, D. A.: Endonuclease II of E. coli. Cold Spr. Harb. Symp. quant. Biol. 33, 271–275 (1968).

    Google Scholar 

  • Holliday, R.: A mechanism for gene conversion in fungi. Genet. Res. (Camb.) 5, 282–304 (1964).

    Google Scholar 

  • Howard-Flanders, P., Boyce, R. P.: DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat. Res., Suppl. 6, 156–184 (1966).

    Google Scholar 

  • Johnston, J. R., Mortimer, R. K.: Use of snail digestive juice in isolation of yeast spore tetrads. J. Bact. 78, 292 (1959).

    Google Scholar 

  • Kaplan, H. S.: DNA-strand scission and loss of viability after X-irradiation of normal and sensitized bacterial cells. Proc. nat. Acad. Sci. (Wash.) 55, 1442–1446 (1966).

    Google Scholar 

  • Manney, T. R., Mortimer, R. K.: Allelic mapping in yeast by X-ray-induced mitotic reversion. Science 143, 581–582 (1964).

    Google Scholar 

  • McGrath, R. A., Williams, R. W.: Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature (Lond.) 212, 534–535 (1966).

    Google Scholar 

  • Reiter, H., Strauss, B.: Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet-sensitive strain of Bacillus subtilis. J. molec. Biol. 14, 179–194 (1965).

    Google Scholar 

  • —, Robbins, M., Marone, R.: Nature of the repair of methyl methanesulfonate-induced damage in Bacillus subtilis. J. Bact. 93, 1056–1062 (1967).

    Google Scholar 

  • Reno, B.: Genetic studies of radiation sensitive mutants of Saccharomyces cerevisiae. M.S. Thesis, University of California, Davis, Library (1969).

  • Searashi, T., Strauss, B.: Relation of the repair of damage induced by a monofunctional alkylating agent to the repair of damage induced by ultraviolet light in Bacillus subtilis. Biochem. biophys. Res. Commun. 20, 680–687 (1965).

    Google Scholar 

  • Strauss, B., Coyle, M., Robbins, M.: Alkylation damage and its repair. Cold Spr. Harb. Symp. quant. Biol. 33, 277–287 (1968).

    Google Scholar 

  • —, Reiter, H., Searashi, T.: Recovery from ultraviolet- and alkylating-agent-induced damage in Bacillus subtilis. Radiat. Res., Suppl. 6, 201–211 (1966).

    Google Scholar 

  • —, Wahl, R.: The presence of breaks in the deoxyribonucleic acid of Bacillus subtilis treated in vivo with the alkylating agent, methyl-methanesulfonate. Biochim. biophys. Acta (Amst.) 80, 116–126 (1964).

    Google Scholar 

  • Wahl, R.: Detection of single strand breaks in DNA alkylated in vitro. Fed. Proc. 24, 226 (1965).

    Google Scholar 

  • Yost, H. T., Chaleff, R. S., Finerty, J. P.: Induction of mitotic recombination in Saccharomyces cerevisiae by ethyl methane sulfonate. Nature (Lond.) 215, 660–661 (1967).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, University of California, Davis, California

    Richard Snow & Christopher T. Korch

Authors
  1. Richard Snow
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christopher T. Korch
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Magni

Rights and permissions

Reprints and permissions

About this article

Cite this article

Snow, R., Korch, C.T. Alkylation induced gene conversion in yeast: Use in fine structure mapping. Molec. Gen. Genet. 107, 201–208 (1970). https://doi.org/10.1007/BF00268694

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00268694

Keywords

Search

Navigation