Abstract
The frequency of hybrid formation between twoTrypanosoma brucei clones during cyclical transmission throughGlossina morsitans centralis was analyzed. In two independent experiments, teneralG. m. centralis were infected with an equal mixture of twoT. brucei clones showing different homozygous isoenzyme patterns for isocitrate dehydrogenase (ICD; E.C. 1.1.1.42) and alkaline phosphatase (AP; E.C. 3.1.3.1). Trypanosomes were cyclically transmitted to mice from 23 infective flies and the subsequent blodstream-form populations were characterized by isoenzyme electrophoresis. Heterozygous patterns for ICD and AP indicated that hybrid formation occurred in at least 9 of the 23 vectors. There was further evidence that extrusion of hybrid parasites with saliva from a single fly was not necessarily continuous but could alter over time with the occurrence of either of both of the homozygous parental clones.
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Schweizer, J., Tait, A. & Jenni, L. The timing and frequency of hybrid formation in African trypanosomes during cyclical transmission. Parasitol Res 75, 98–101 (1988). https://doi.org/10.1007/BF00932707
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DOI: https://doi.org/10.1007/BF00932707