Summary
The early morphogenesis of the eye-antennal disc ofDrosophila in response to 20-hydroxy ecdysone involves the curling of the eye anlagen dorsally over the antenna. During this process, the area of the peripodial membrane is substantially reduced. The peripodial membrane is taut at this stage, and if it is cut the curling of the disc cannot continue, and the eye anlagen returns to its original position within one minute of the operation. In contrast, cutting the columnar epithelium between the eye and antennal anlagen does not disrupt curling, but actually facilitates it. During curling, the cells of the peripodial membrane appear healthy, and exhibit basal extensions. We suggest that the curling of the eye is mediated by the conversion of cuboidal peripodial membrane cells into pseudostratified columnar epithelium at the edges of the peripodial membrane. Subsequently, cells of the peripodial membrane secrete first a pupal cuticle, and then an imaginal cuticle.
Similar content being viewed by others
References
Bryant PJ (1975) Pattern formation in the imaginal wing disc ofDrosophila melanogaster: Fate map, regeneration and duplication J Exp Zool 193:49–78
Bryant PJ (1978) Pattern formation in imaginal discs. In: Ashburner M, wright TRF (eds) The genetics and biology ofDrosophila. Academic Press, New York and London, pp 229–335
Deak II (1980) A model linking segmentation, compartmentalization and regeneration inDrosophila development. J Theor Biol 84:477–504
Edwards JS, Milner MJ, Chen SW (1978) Integument and sensory nerve differentiation ofDrosophila leg and wing imaginal discs in vitro. Wilhelm Roux's Arch 185:59–77
Gottschewski GHM (1960) Morphogenetische Untersuchungen an in vitro wachsenden Augenanlagen vonDrosophila melanogaster. Wilhelm Roux's Arch 152:204–229
Haynie JL (1975) Ph.D. Thesis, University of California, Irvine
Karpen GH, Schubiger G (1981) Extensive regulatory capabilities of aDrosophila imaginal disk blastema. Nature 294:744–747
Kumar K, Ouweneel WJ, Faber J (1979) Differentiation capacities of the labial imaginal disc ofDrosophila melanogaster. Wilhelm Roux's Arch 186:51–64
Locke M (1981) Cell structure during insect metamorphosis. In: Gilbert LI, Frieden E (eds) Metamorphosis: a problem in developmental biology. Plenum Press, New York, pp 75–103
Locke M, Huie P (1981) Epidermal feet in pupal segment morphogenesis. Tissue Cell 13:7870803
Milner MJ (1980) Epithelial and pattern integration inDrosophila eye-antennal imaginal discs cultured in vitro. In: Kurstak E, Maramorosch K, Dübendorfer A (eds) Invertebrate systems in vitro. Elsevier/North Holland Biomedical Press Amsterdam-Oxford-New York pp 135–148
Milner MJ, Haynie JL (1979) Fusion ofDrosophila eye-antennal imaginal discs during differentiation in vitro. Wilhelm Roux's Arch 185:363–370
Milner MJ, Sang JH (1974) Relative activities of α-ecdysone and β-ecdysone for the differentiation in vitro ofDrosophila melanogaster imaginal discs. Cell 3:141–143
Ouweneel WJ (1970) Developmental capacities of young and mature, wild-type and opht eye imaginal discs inDrosophila melanogaster. Wilhelm Roux's Arch 166:78–88
Poodry CA, Schneiderman HA (1970) The ultrastructure of the developing leg ofDrosophila melanogaster. Wilhelm Roux's Arch 166:1–44
Ready DF, Hanson TE, Benzer S (1976) Development of theDrosophila retina, a neurocrystalline lattice. Dev Biol 53:217–240
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Robertson CW (1936) The metamorphosis ofDrosophila melanogaster, including an accurately timed account of the principle morphological changes. J Morphol 59:351–399
Schneider I (1964) Differentiation of larvalDrosophila eye-antennal discs in vitro. J Exp Zool 156:91–104
Schneider I (1966) Histology of larval eye-antennal disks and cephalic ganglia ofDrosophila cultured in vitro. J Embryol Exp Morphol 15:271–279
Shields G, Sang JH (1977) Improved medium for culture ofDrosophila embryonic cells. Drosophila Inform Serv 52:161
Sprey TE (1970) Morphological and histochemical changes during the development of some of the imaginal disks ofCalliphora erythrocephala. Neth J Zool 20:253–275
Sprey TE, Oldenhave M (1974) A detailed organ map of the wing disk ofCalliphora erythrocephala. Neth J Zool 24:291–310
Tucker JB (1967) Changes in nuclear structure during binary fission in the ciliateNassula. J Cell Sci 2:481–498
Ursprung H (1972) The fine structure of imaginal discs. In: Ursprung H, Nöthiger R (eds) The biology of imaginal discs. Springer, Berlin Heidelberg New York, pp 93–107
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Milner, M.J., Bleasby, A.J. & Pyott, A. The role of the peripodial membrane in the morphogenesis of the eye-antennal disc ofDrosophila melanogaster . Wilhelm Roux' Archiv 192, 164–170 (1983). https://doi.org/10.1007/BF00848686
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00848686