Summary
The total amount of ATP and UTP was measured in embryos ofDrosophila melanogaster andBruchidius obtectus at different development stages. The pool-sizes were measured by their ability to enhance poly(AU) synthesis in an in vitro test, using RNA polymerase ofE. coli, poly-d(AT), ATP, and3H-UTP. In embryos ofD. melanogaster, the amount of UTP decreased from 4.5 pmoles/ embryo during cleavage and early blastoderm to 2 pmoles at later stages. The ATP pool decreased from 14 pmoles/ embryo to 6 pmoles at the same developmental stages. In embryos ofB. obtectus, the UTP pool expanded from about 1 pmole/embryo during cleavage and early blastoderm to about 3 pmoles during gastrulation and organogenesis. Much more ATP was found inB. obtectus embryos: during fertilization 23 pmoles/embryo were found. At late syncytial blastoderm stages the amount of ATP rose to about 50 pmoles/embryo. At cellular blastoderm stages and during gastrulation only 26 pmoles/embryo of ATP were found. During organogenesis the ATP pool decreased to about 14 pmoles/embryo.
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References
Anderson KV, Lengyel JA (1979) Rates of synthesis of major, classes of RNA inDrosophila embryos. Dev Biol 70:217–231
Anderson KV and Lengyel JA (1981) Changing rates of DNA and RNA synthesis inDrosophila embryos. Dev Biol 82:127–138
Büning J (1978) Changes in RNA synthesis of endogenous “free” and template-engaged RNA polymerases in isolated nuclei of highly synchronized coleopteran embryos. Dev Biol 64:130–139
Büning J (1980) RNA synthesis during early embryogenesis of mass cultured highly synchronized coleopteran embryos. Wilhelm Roux' Arch 188:215–224
Davidson E (1976) Gene activity in early development. 2nd edition. Academic Press, New York
Fausto-Sterling A, Zheutlin LM, Brown PR (1974) Rates of RNA synthesis during early embryogenesis inDrosophila melanogaster. Dev Biol 40:78–83
Jamieson AT, Webb DR (1976) A sensitive enzymatic determination of ribonucleoside triphosphates in cell extracts. Anal Biochem 72:212–219
Jung E (1966) Untersuchungen am Ei des SpeisebohnenkäfersBruchidius obtectus Say. (Coleoptera). I. Mitteilung: Entwicklunesgeschichtliche Ergebnisse zur Kennzeichnung des Eitypus. Z Morphol Ökol Tiere 56:444–480
King RC (1963) Trends towards ovoviviparity inDrosophila. Dros Inf Serv 38:96
Rickoll WL (1976) Cytoplasmic continuity between embryonic cells and the primitive yolk sac during early gastrulation inDrosophila melanogaster. Dev Biol 49:304–310
Sasvári-Székely M, Vitéz M, Staub M, Antoni F (1975) Determination of UTP and ATP pool sizes in human tonsilla lymphocytes by usingEscherichia coli RNA polymerase. Biochim Biophys Acta 395:221–228
Schrankel KR, Schwalm FE (1981) Quantification of ribonucleotides and uridine metabolism in ovaries of the kelp fly (Coelopa frigida, Diptera) during final stages of oogenesis. Insect Biochem 11:117–120
Sonnenblick BP (1950) The early embryology ofDrosophila melanogaster. In: Demerec M (ed) Biology of Drosophila. Wiley, New York, pp 162–267
Ulrich H (1953) A convenient method for collecting large numbers ofDrosophila eggs homogenous in age. Dros Inf Serv 27:125–127
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Büning, J. Changes of RNA precursor pools (ATP, UTP) during embryogenesis ofDrosophila melanogaster (Diptera) andBruchidius obtectus (coleoptera). Wilhelm Roux' Archiv 192, 159–163 (1983). https://doi.org/10.1007/BF00848685
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DOI: https://doi.org/10.1007/BF00848685