Skip to main content
SpringerLink
Log in

Effects of cycloheximide on cellular elongation in aManduca sexta cell line

  • Rapid Communication
  • Published:
Wilhelm Roux's archives of developmental biology Aims and scope Submit manuscript

Summary

A clone (GV1) of the CHIManduca sexta cell line responds to 20-hydroxy-ecdysone by changing cellular shape from an epithelial-like form to an elongated fibroblast-like form. We have determined that this morphological response to hormone is prevented by treatment with cycloheximide. The inhibition of the elongation response by cycloheximide may relate to a requirement for the synthesis of specific proteins that play a role in the formation of cytoskeletal structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Berger E, Ringler R, Alahiotis S, Frank M (1978) Ecdysone-induced changes in morphology and protein synthesis inDrosophila cell cultures. Dev Biol 62:498–511

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Cherbas P, Cherbas L, Williams CM (1977) Induction of acetylcholinesterase activity by β-ecdysone in aDrosophila cell line. Science 197:275–278

    Article  CAS  Google Scholar 

  • Courgeon A-M (1972) Action of insect hormones at the cellular level Exp Cell Res 74:327–336

    Article  CAS  Google Scholar 

  • Lynn DE, Oberlander H (1980) The role of microtubules in the response of a cloned insect cell line to 20-hydroxyecdysone. In Vitro 16:223

    Google Scholar 

  • Marks EP, Holman GM (1979) Ecdysone action on insect cell lines. In Vitro 15:300–307

    Article  CAS  Google Scholar 

  • Oberlander H (1976) Hormonal control of growth and differentiation of insect tissues cultured in vitro. In Vitro 12:225–235

    Article  CAS  Google Scholar 

  • Oberlander H (1980) Morphogenesis in tissue culture: Control by ecdysteroids. In: Locke M, Smith DD (eds) Insect biology in the future (in press). Academic Press, New York

    Google Scholar 

  • Oberlander H, Ferkovich S, Leach E, Essen F Van (1980) Inhibition of chitin biosynthesis in cultured amiginal discs: Effects of alpha-amanitin, actinomycin D, cycloheximide, and puromycin. Wilhelm Roux's Archives 188:81–86

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Insect Attractants, Behavior and Basic Biology Research Laboratory, Agricultural Research, Science and Education Administration, USDA, 32604, Gainesville, Florida, USA

    H. Oberlander & C. E. Leach

  2. Department of Entomology, University of Florida, 32611, Gainesville, Florida, USA

    D. E. Lynn

Authors
  1. H. Oberlander
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. E. Leach
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. E. Lynn
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Employed through a cooperative agreement with the Insect Attractants, Behavior and Basic Biology Research Laboratory

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oberlander, H., Leach, C.E. & Lynn, D.E. Effects of cycloheximide on cellular elongation in aManduca sexta cell line. Wilhelm Roux' Archiv 190, 60–61 (1981). https://doi.org/10.1007/BF00868706

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00868706

Key words

Search

Navigation