Summary
In conidia-free submerged cultures of Neurospora crassa the various steps of light-dependend carotenoid synthesis were studied. The mycelium produces small amounts of pigments even in the dark. The data obtained are in part in good agreement with earlier results of Zalokar and show that the light-induced pigment production starts after a lag-period of 40 min and is finished after 6–8 hours; the photoreaction is saturated by relatively small dosages. In contrast to Zalokar's results we found that for photoinduction the reciprocity law holds true. The photoreaction is saturated by a certain amount of light independently of the light-intensity. Actidion (Cycloheximide) inhibits carotenoid synthesis completely when added before or up to 10 min after the onset of illumination, whereas addition 60 min after illumination already has no effect. Comparison with the results obtained with Fusarium shows that the reaction mechanism is very similar in both organisms, though the various steps seem to proceed faster in Neurospora.
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Herrn Prof. Dr. L. Brauner in Verehrung und Dankbarkeit zum 70. Geburtstag gewidmet.
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Rau, W., Lindemann, I. & Rau-Hund, A. Untersuchungen über die lichtabhängige Carotinoidsynthese. Planta 80, 309–316 (1968). https://doi.org/10.1007/BF00392400
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DOI: https://doi.org/10.1007/BF00392400