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Lipid-body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms

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Abstract

Lipid bodies are degraded during germination. Whereas some proteins, e.g. oleosins, are synthesized during the formation of lipid bodies of maturating seeds, a new set of proteins, including a specific form of lipoxygenase (LOX; EC 1.13.11.12), is detectable in lipid bodies during the stage of fat degradation in seed germination. In cotyledons of cucumber (Cucumis sativus L.) seedlings at day 4 of germination, the most conspicuous staining with anti-LOX antibodies was observed in the cytosol. At very early stages of germination, however, the LOX form present in large amounts and synthesized preferentially was the lipid-body LOX. This was demonstrated by immunocytochemical staining of cotyledons from 1-h and 24-h-old seedlings: the immunodecoration of sections of 24-h-old seedlings with anti-LOX antiserum showed label exclusively correlated with lipid bodies of around 3 μm in diameter. In accordance, the profile of LOX protein isolated from lipid bodies during various stages of germination showed a maximum at day 1. By measuring biosynthesis of the protein in vivo we demonstrated that the highest rates of synthesis of lipid-body LOX occurred at day 1 of germination. The early and selective appearance of a LOX form associated with lipid bodies at this stage of development is discussed.

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Abbreviations

DAPI:

4,6-diamidino-2-phenylindole

DIC:

differential interference contrast

FITC:

fluorescein-5-isothiocyanate

LOX:

lipoxygenase

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Author notes

  1. Ivo Feussner

    Present address: Institut für Pflanzenbiochemie, POB 250, D-06018, Halle, Germany

Authors and Affiliations

  1. Fachbereich Chemie, Philipps-Universität, D-35032, Marburg, Germany

    Ivo Feussner, Arndt Nellen & Helmut Kindl

  2. Institut für Pflanzenbiochemie, Weinberg 3, D-06120, Halle, Germany

    Ivo Feussner, Bettina Hause & Claus Wasternack

Authors
  1. Ivo Feussner
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  2. Bettina Hause
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  3. Arndt Nellen
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  4. Claus Wasternack
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  5. Helmut Kindl
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Additional information

This work was financially supported by the Deutsche Forschungsgemeinschaft, SFB 286 (I.F., A.N., H.K.) and SFB 363 (B.H., C.W.).

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Feussner, I., Hause, B., Nellen, A. et al. Lipid-body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms. Planta 198, 288–293 (1996). https://doi.org/10.1007/BF00206255

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  • DOI: https://doi.org/10.1007/BF00206255

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