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Stimulation by auxin of phospholipase A in membrane vesicles from an auxin-sensitive tissue is mediated by an auxin receptor

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Abstract

Microsomal vesicles were prepared from zucchini (Cucurbita pepo L.) hypocotyls containing radioactive phosphatidylethanolamine or phosphatidylcholine, and these lipids were used as substrates by phospholipase A which is activated by auxins. Phospholipase D and phospholipase C hydrolysed the same substrates but were not influenced by auxin. Phospholipase A was activated by the auxins indolyl-3-acetic acid, 2,4-dichlorophenoxyacetic acid and, to a lesser extent, by α-naphthaleneacetic acid whereas the weak auxins 2,3-dichlorophenoxyacetic acid and β-naphthaleneacetic acid were almost inactive. This hormone specificity was also found in growth tests with etiolated zucchini hypocotyls. Phospholipase A activation by auxin was blocked by a polyclonal antibody against the maize auxin-binding protein. We propose that phospholipase A activation is a primary reaction in the signal transduction leading from hormone-binding to the growth response.

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Abbreviations

IAA:

indolyl-3-acetic acid

2,3-D, 2,4-D:

2,3- and 2,4-dichlorophenoxyacetic acid

α-NAA; β-NAA:

α- and β-naphthaleneacetic acid

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  1. Botanisches Institut, Universität Bonn, Venusbergweg 22, 1, Bonn, Germany

    Bernadette André & Günther F. E. Scherer

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  1. Bernadette André
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  2. Günther F. E. Scherer
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This work was supported by the Deutsche Forchungsgemeinschaft. We thank D. Klämbt (Botanical Institute, University of Bonn, FRG) for a generous gift of polyclonal antibody (IgG fraction) against auxin-binding protein and U. Kutschera (Botanical Institute, University of Bonn, FRG) for advice with the growth tests.

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André, B., Scherer, G.F.E. Stimulation by auxin of phospholipase A in membrane vesicles from an auxin-sensitive tissue is mediated by an auxin receptor. Planta 185, 209–214 (1991). https://doi.org/10.1007/BF00194062

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  • DOI: https://doi.org/10.1007/BF00194062

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