Abstract
The mechanisms underlying the response of the mature barley (Hordeum vulgare L.) aleurone layer to gibberellic acid have received much attention, but little is known about the developmental basis for this response. We have investigated the spatial and temporal accumulation of mRNAs complementary to two barleygrain cDNAs that are differentially expressed in the aleurone layer of the developing endosperm. Messenger RNA complementary to one of these clones (B11E; Jakobsen etal., 1989, Plant Mol. Biol. 12, 285–293) accumulates exclusively in the aleurone layer of developing grains where it is uniformly distributed in all three cell layers. Accumulation of B11E mRNA is first detectable 10 d post an thesis (DPA), increases 200-fold up to 25 DPA, and then declines towards grain maturity. Messenger RNA complementary to the other clone, B22E, shows a more complex pattern of expression. In addition to the aleurone layer, this mRNA accumulates in the vascular tissue of the maternal pericarp and embryo axis, as well as in the parenchyma cells of the embryonic scutellum. In excised immature embryos abscisic acid strongly suppresses accumulation of B22E mRNA. The B22E transcript is absent from mature embryos, but rapidly reappears after germination.
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Abbreviations
- ABA:
-
abscisic acid
- DPA:
-
days post anthesis
- GA3 :
-
gibberellic acid
- kb:
-
kilobase
References
Bosnes, M., Harris, E., Aigeltinger, L., Olsen, O.-A. (1987) Morphology and ultrastructure of 11 barley shrunken endosperm mutants. Theor. Appl. Genet. 74, 177–187
Chandler, V.L., Maler, B.A., Yamamoto, K.R. (1983) DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter responsive in vivo. Cell 33, 489–499
Galau, G.A., Legocki, A.B., Greenway, S.C., Dure, L., III (1981) Cotton messenger RNA sequences exist in both polyadenylated and nonpolyadenylated forms. J. Biol. Chem. 256, 2551–2560
Galau, G.A., Hughes, D.W., Dure, L. (1986) Abscisic acid induction of cloned cotton late embryogenesis-abundant (Lea) mRNAs. Plant Mol. Biol. 7, 155–170
Galau, G.A., Bijaisoradat, N., Hughes, D.W. (1987) Accumulation kinetics of cotton late embryogenesis-abundant mRNAs and storage protein mRNAs: Coordinate regulation during embryogenesis and the role of abscisic acid. Dev. Biol. 123, 198–212
Gomez, J., Sanchez-Martinez, D., Steifel, V., Rigau, J., Puigdomenech, P., Pages, M. (1988) A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334, 262–264
Hughes, D.W., Galau, G.A. (1989) Temporally modular gene expression during cotyledon development. Genes Dev. 3, 358–369
Jakobsen, K.S. (1989) Gene activity in the developing barley grain: A study of spatial and temporal expression using cloned mRNAs. Ph. D. thesis, Dept. of Biology, University of Oslo, Norway
Jakobsen, K.S., Klemsdal, S.S., Aalen, R.B., Bosnes, M., Alex-ander, D., Olsen, O.-A. (1989) Barley aleurone cell development: molecular cloning of aleurone specific cDNAs from immature grains. Plant Mol. Biol. 12, 285–293
Klemsdal, S.S., Kvaale, A., Olsen, O.-A. (1986) Effects of the barley mutants Risø 1508 and 527 high lysine genes on the cellular development of the endosperm. Physiol. Plant. 67, 453–459
Lin, L.S., Ho, D.H.T. (1986) Mode of action of abscisic acid in barley aleurone layers: induction of new proteins by abscisic acid. Plant Physiol. 82, 289–297
Marcotte, W.R., Bayley, C.C., Quatrano, R.S. (1988) Regulation of a wheat promoter by abscisic acid in rice protoplasts. Nature 335, 454–457
Mundy, J., Chua, N.-H. (1988) Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 7, 2279–2286
Mundy, J., Rogers, J.C. (1986) Selective expression of a probable amylase/protease inhibitor in barley aleurone cells: Comparison to the barley amylase/subtilisin inhibitor. Planta 169, 51–63
Mundy, J., Hejgaard, J., Hansen, A., Hallgren, L., Jørgensen, K.G., Munck, L. (1986) Differential synthesis in vitro of barley aleurone and starchy endosperm proteins. Plant Physiol. 81, 630–636
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497
Rogers, J.C. (1985) Two barley α-amylase gene families are regulated differently in aleurone cells. J. Biol. Chem. 260, 3731–3738
Sakai, D.D., Helms, S., Carlstedt-Duke, J., Gustafsson, J.-Å., Rottman, F.M., Yamamoto, K.R. (1988) Hormone-mediated repression: a negative glucocorticoid response element from the bovine prolactin gene. Genes Dev. 2, 1144–1154
Schmelzer, E., Jahnen, W., Hahlbrock, K. (1988) In situ localization of light-induced chalcone synthetase mRNA, chalcone synthetase, and flavonoid end products in epidermal cells of parsley leaves. Proc. Natl. Acad. Sci. USA 85, 2989–2993
Somssich, I.E., Schmelzer, E., Kawalleck, P., Hahlbrock, K. (1988) Gene structure and in situ transcript localization of pathogene-sis-related protein 1 in parsley. Mol. Gen. Genet. 213, 93–98
Williamson, J.D., Quatrano, R.S., Cuming, A.C. (1985) Empolypeptide and its messenger RNA levels are modulated by ABA during embryogenesis in wheat. Eur. J. Biochem. 152, 501–507
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John Mundy (Department of Biotechnology, Carlsberg Research Center, Copenhagen, Denmark) is thankfully acknowledged for critically reading the manuscript, and Solveig H. Engebretsen for skillful technical assistance.
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Olsen, OA., Jakobsen, K.S. & Schmelzer, E. Development of barley aleurone cells: temporal and spatial patterns of accumulation of cell-specific mRNAs. Planta 181, 462–466 (1990). https://doi.org/10.1007/BF00192998
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DOI: https://doi.org/10.1007/BF00192998