Abstract
The fungal phytotoxin tentoxin and its natural derivative dihydrotentoxin impair light-induced stomatal opening in epidermal strips of broad bean (Vicia faba L.) incubated in a potassium-rich medium. Swelling of guard-cell protoplasts (GCPs) of the same species is inhibited in the presence of both substances. Swollen GCPs shrink after tentoxin or dihydrotentoxin treatment and these effects cannot be fully compensated by the phytoeffector fusicoccin. A comparison with the potassium carrier valinomycin shows that tentoxin acts in a different manner, because it is effective in the light only, whereas valinomycin causes shrinkage of GCPs also in the dark. Determination of adenine nucleotides in GCPs indicates a reduced ATP content and an enhanced ADP level after addition of tentoxin. At the same time, tentoxintreated GCPs contain more NADPH and less NAD+ than the control (NADP+ and NADH content does not differ). The results presented are consistent with the hypothesis that tentoxin closes stomata as a consequence of its inhibitory action on photophosphorylation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- FC:
-
fusicoccin
- GCP:
-
guard-cell protoplast
- KIDA:
-
potassium iminodiacetate
References
Arntzen, C.J. (1972) Inhibition of photophosphorylation by tentoxin, a cyclic tetrapeptide. Biochim. Biophys. Acta 283, 539–542
Bulychev, A.A., Dahse, J. (1984) Light-induced electric potentials of intact Anthoceros chloroplasts and their modification in the presence of the energy transfer inhibitor tentoxin. Biochem. Physiol. Pflanz. 179, 685–692
Dahse, I., Bulychev, A.A., Kurella, G.A., Liebermann, B., (1985) Weak tentoxin effect on the electrical light response of isolated chloroplasts of Peperomia metallica. Physiol. Plant. 65, 446–450
Dahse, I., Felle, H., Bentrup, F.-W., Liebermann, B. (1986a) Tentoxin and valinomycin affect electrophysiological properties and K+ (86Rb+) fluxes in the liverwort Riccia fluitans. J. Plant Physiol. 124, 87–93
Dahse, I., Matorin, D.N., Liebermann, B. (1986b) A comparison of tentoxin action on the delayed fluorescence in chloroplasts of spinach, Chlorella and Anacystis. Biochem. Physiol. Pflanzen 181, 137–146
Dahse, I., Müller, E., Liebermann, B., Eichhorn, M. (1987) The membrane potential as indicator for transport and energetic processes of leaf cells of the aquatic plant Egeria densa. II. The effect of the specific energy transfer inhibitor tentoxin and its derivative dihydrotentoxin. Biochem. Physiol. Pflanzen (in press)
Duke, S.O., Paul, R.N., Wickliff, J.L. (1980) Tentoxin effects on ultrastructure and greening in ivyleaf morining glory (Ipomoea hederacea var. hederacea) cotyledons. Physiol. Plant. 49, 27–36
Duke, S.O., Wickliff, J.L., Vaughn, K.C., Paul, R.N. (1982) Tentoxin does not cause chlorosis in greening mung bean leaves by inhibiting photophosphorylation. Physiol. Plant. 56, 387–398
Durbin, R.D., Uchytil, T.F. (1972) The effect of tentoxin on transpiration. (Abstr.) Phytopathology 62, 755
Durbin, R.D., Uchytil, T.F. (1976) The effect of tentoxin on fusicocein-induced stomatal opening. Phytopathol. Mediterr. 15, 62–63
Durbin, R.D., Uchytil, T.F. (1977) A survey of plant insensitivity of tentoxin. Phytopathology 67, 602–603
Durbin, R.D., Uchytil, T.F., Sparapano, L. (1973) The effect of tentoxin on stomatal aperture and potassium content of guard cells. Phytopathology 63, 1077–1078
Hampp, R., Goller, M., Füllgraf, H. (1984) Determination of compartmented metabolite pools by a combination of rapid fractionation of oat mesophyll protoplasts and enzymic cycling. Plant Physiol. 75, 1017–1021
Hampp, R., Goller, M., Ziegler, H. (1982) Adenylate levels, energy charge, and phosphorylation potential during dark/light and light/dark transition in chloroplasts, mitochondria and cytosol of mesophyll protoplasts from Avena sativa L. Plant Physiol. 69, 448–455
Hampp, R., Schnabl, H. (1984) Adenine and pyridine nucleotide status of isolated Vicia guard cell protoplasts during K+-induced swelling. Plant Cell Physiol. 25, 1233–1239
Ishikawa, H., Aizawa, H., Kishira, H., Ogawa, T., Sakata, M. (1983) Light-induced changes of membrane potential in guard cells of Vicia faba. Plant Cell Physiol. 24, 769–772
Klotz, M.G., Gündel, J., Müller, E., Liebermann, B. (1982) Tentoxin action on the electric properties of a lipid bilayer membrane. Stud. Biophys. 90, 53–54
Lowry, O.H., Passonneau, J.V. (1972) A flexible system of enzymatic analysis. Academic Press, New York
Lüttge, U., Clarkson, D.T. (1985) II. Mineral nutrition: Plasmalemma and tonoplast redox activities. Prog. Bot. 47, 73–86
Marré, E. (1980) Mechanism of action phytotoxins affecting plasmalemma functions. Prog. Phytochem. 6, 253–284
Müller, E., Klotz, M., Dahse, I. (1984) Tentoxin — a phytotoxic high-selective K+-carrier. In: Membrane transport in plants, pp 503–504, Cram W.J., Janacek, K., Rybova, R., Sigler, K., eds. Academia, Praha
Raschke, K. (1975) Stomatal action. Annu. Rev. Plant Physiol. 26, 309–340
Schnabl, H., Bornman, C., Ziegler, H. (1978) Studies in isolated starch-containing and starch-deficient guard cell protoplasts. Planta 143, 33–39
Schwartz, A., Zeiger, E. (1984) Metabolic energy for stomatal opening. Roles of photophosphorylation and oxidative phosphorylation. Planta 161, 129–136
Steele, J.A., Uchytil, T.F., Durbin, R.D., Bhatnagar, P.K., Rich, D.H. (1976) Chloroplast coupling factor 1: A speciesspecific receptor for tentoxin. Proc. Natl. Acad. Sci. USA 73, 2245–2248
Templeton, G.E. (1972) Alternaria toxins related to Pathogenesis in plants. In: Microbial toxins, vol. 8, pp. 169–192, Kadis, S., Ciegler, A., Ajl, S.J. eds. Academic Press, New York London
Templeton, G.E., Grable, C.J., Fulton, N.D., Bollenbacher, K. (1967) Factors affecting the amount and pattern of chlorosis caused by a metabolite of Alternaria tenuis. Phytopathology 57, 516–518
Weyers, J.D.B., Fitzsimons, P.J., Mansey, G.M., Martin, E.S. (1983) Guard cell protoplasts — aspects of work with an important new research tool. Physiol. Plant. 58, 331–339
Zeiger, E., Hepler, P.K. (1976) Production of guard cell protoplasts from onion and tobacco. Plant Physiol. 58, 429–498
Zeiger, E., Hepler, P.K. (1977) Light and stomatal function: blue light stimulates swelling of guard cell protoplasts. Science 196, 886–889
Ziegler, H., Pearl Lin Hsiu-Ping, Schnabl, H. (1981) Änderungen in der intra- und intereellulären Kompartimentierung. Ber. Deutsch. Bot. Ges. 94, 193–202
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dahse, I., Schnabl, H., Hampp, R. et al. Inhibition of light-induced stomatal opening and of guard-cell-protoplast swelling in Vicia faba L. by tentoxin, an inhibitor of photophosphorylation. Planta 173, 391–396 (1988). https://doi.org/10.1007/BF00401026
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00401026