Abstract
The assimilation of nitrate under dark-N2 and dark-O2 conditions in Zea mays leaf tissue was investigated using colourimetric and 15N techniques for the determination of organic and inorganic nitrogen. Studies using 15N indicated that nitrate was assimilated under dark conditions. However, the rate of nitrate assimilation in the dark was only 28% of the rate under non-saturating light conditions. No nitrite accumulated under dark aerobiosis, even though nitrate reduction occurred under these conditions. The pattern of nitrite accumulation in leaf tissue in response to dark-N2 conditions consisted of three phases: an initial lag phase, followed by a period of rapid nitrite accumulation and finally a phase during which the rate of nitrite accumulation declined. After a 1-h period of dark-anaerobiosis, both nitrate reduction and nitrite accumulation declined considerably. However, when O2 was supplied, nitrate reduction was stimulated and the accumulated nitrite was rapidly reduced. Anaerobic conditions stimulated nitrate reduction in leaf tissue after a period of dark-aerobic pretreatment.
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Ariovich, D., Cresswell, C.F. (1983) The effect of nitrogen and phosphorus on starch accumulation and net photosynthesis in two variants of Panicum maximum Jacq. Plant Cell Environ. 6, 657–664
Aslam, M., Huffaker, R.C., Rains, D.W., Rao, R.P. (1979) Influence of light and ambient carbon dioxide concentration on nitrate assimilation by intact barley seedlings. Plant Physiol. 63, 1205–1209
Beevers, H. (1961) Respiratory metabolism in plants. Row, Peterson and Co., Evanston Illinois, White Plains, New York
Ben-Shalom, N., Huffaker, R.C., Rappaport, L. (1983) Effect of photosynthetic inhibitors and uncouplers of oxidative phosphorylation on nitrate and nitrite reduction in barley leaves. Plant Physiol. 71, 63–66
Bremmer, J.M. (1965) Inorganic forms of Nitrogen. In: Methods of soil analysis, vol. 9, pt. 2, pp. 1179–1237, Black, C.A., Evans, D.D., White, J.L., Ensminger, L.E., Clark, F.E., eds., Am. Soc. Agron., Inc., Publisher Madison, Wisconsin, USA
Canvin, D.T., Woo, K.C. (1979) The regulation of nitrate reduction in spinach leaves. Can. J. Bot. 57, 1155–1160
Cataldo, D.A., Haroon, M., Schrader, L.E., Youngs, V.L. (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun. Soil Sci. Plant Anal. 6, 71–80
Chaparro, A., Maldonado, J.M., Diez, J., Relimpio, A.M., Losada, M. (1976) Nitrate reductase inactivation and reducing power and energy charge in Chlorella cells. Plant Sci. Lett. 6, 335–342
Dry, I., Wallace, W., Nicholas, D.J.D. (1981) Role of ATP in roots of wheat and pea. Planta 152, 234–238
Eaglesham, A.R.J., Hewitt, E.J. (1975) Inhibition of nitrate reductase from spinach (Spinacea oleracea) leaf by adenosine nucleotides. Plant Cell Physiol. 16, 1137–1149
Gray, V.M., Cresswell, C.F. (1984) The effect of inhibitors of photosynthetic and respiratory electron transport on nitrate reduction and nitrite accumulation in excised Z. mays L. leaves. J. Exp. Bot. 35, 1166–1176
Hageman, R.H., Reed, A.J. (1980) Nitrate reductase from higher plants. Methods Enzymol. 69, 273–274
Hewitt, E.J. (1952) Sand and water culture methods used in the study of plant nutrition. Tech. Commun. No. 22, Common-wealth Bureau of Horticulture and Plantation Crops, East Malling, Kent
Kato, T. (1980) Nitrogen assimilation by a citrus tree. 2. Assimilation of labelled ammonium and nitrate by detached leaves in light and dark. Physiol. Plant. 50, 304–308
Klepper, L., Flesher, D., Hageman, R.H. (1971) Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiol. 48, 580–590
Kow, Y.W., Erbes, D.L., Gibbs, M. (1982) Chloroplast respiration. A means of supplying oxidized pyridine nucleotide for dark chloroplastic metabolism. Plant Physiol. 69, 442–447
Krebs, H.A. (1972) The Pasteur effect and the relations between respiration and fermentation. In: Essays in biochemistry, vol. 8, 1–34, Campbell, P.N., Dickens, F., ed., Academic Press, London New York
Mann, A.F., Hucklesby, D.P., Hewitt, E.J. (1978) Sources of reducing power for nitrate reduction in spinach leaves. Planta 140, 261–263
Miflin, B.J. (1974) The role of light in nitrite reduction; studies with leaf discs. Planta 105, 225–233
Nelson, N., Ilan, I. (1969) Inhibition of nitrate reductase from tomato leaves by adenosine-5′-diphosphate. Plant Cell Physiol. 10, 143–148
Sawhney, S.K., Naik, M.S., Nicholas, D.J.D. (1978) Regulation of nitrate reduction by light, ATP and mitochondrial respiration in wheat leaves. Nature 272, 647–648
Soares, M.I., Lips, S.H., Cresswell, C.F. (1985) Regulation of nitrate and nitrite reduction in barley leaves. Physiol. Plant. 64, 492–500
Turner, J.F., Turner, D.H. (1980) The regulation of glycolysis and the pentose phosphate pathway. In: The biochemistry of plants, vol. 2, pp. 279–312, Davis, D.D. ed., Academic Press, New York London Toronto
Vaugh, K.D., Duke, S.O. (1981) Histochemical localization of nitrate reductase. Histochemistry 72, 191–198
Vaugh, K.C., Duke, S.O., Funkhouser, E.A. (1984) Immunochemical characterization and localization. Physiol. Plant. 62, 481–484
Woo, K.C., Canvin, D.T. (1980) The role of malate in nitrate reduction in spinach leaves. Can. J. Bot. 58, 517–521
Yoneyama, T. (1984) 15N study on the dark assimilatory reduction of nitrate and nitrite in the leaf sections of chlorophyll mutants of rice (Oryza sativa L.). Plant Sci. Lett. 33, 195–200
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Watt, M.P., Gray, V.M. & Cresswell, C.F. The effect of oxygen on nitrate and nitrite assimilation in leaves of Zea mays L. under dark conditions. Planta 169, 339–344 (1986). https://doi.org/10.1007/BF00392129
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DOI: https://doi.org/10.1007/BF00392129