Abstract
Chlamydomonas eugametos gametes agglutinate via the surfaces of their flagella. The mating-type minus (mt -) agglutination factor is a high-molecular-weight glycoprotein called PAS-1.2, present on the exterior surface of the flagellar membrane. During flagellar regeneration, mt - gametes were able to agglutinate as soon as the flagella protruded as short stumps. This was also observed when protein synthesis was blocked, indicating that gametes possess a pool of PAS-1.2. When the exterior surface of flagella-less gametes was extracted and the proteins were subjected to gel electrophoresis, large quantities of PAS-1.2 were detected. Using anti-PAS-1.2 serum, the presence of PAS-1.2-like material was visualized on the plasma membrane of mt - gamete cell bodies. By assaying the biological activity of extracts of the cell bodies and of isolated flagella, it was calculated that the plasma membrane of the cell bodies contains 25 times the activity present in the flagella and could, therefore, represent a large pool of mt - agglutination factor.
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Abbreviations
- mt +/- :
-
mating type plus or minus
- PAS:
-
Periodic-acid-Schiff reagent
References
Goodenough, U.W., Detmers, P.A., Hwang, C. (1982) Activation for cell fusion in Chlamydomonas: analysis of wild-type gametes and non-fusing mutants. J. Cell Biol. 92, 378–386
Homan, W.L., Musgrave, A., Molenaar, E.M., van den Ende, H. (1980) Isolation of monovalent sexual binding components from Chlamydomonas flagellar membranes. Arch. Microbiol. 128, 120–125
Jaenicke, L., Waffenschmidt, S. (1981) Liberation of reproductive units in Volvox and Chlamydomonas: proteolytic processes. Ber. Dtsch. Bot. Ges. 94, 375–386
Laemmlli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680–685
Lefebvre, P.A., Nordstrom, S.A., Moulder, J.E., Rosenbaum, J.L. (1978) Flagellar elongation and shortening in Chlamydomonas. IV. Effects of flagellar detachment, regeneration and resorption on the induction of flagellar protein synthesis. J. Cell Biol. 78, 8–27
Lens, P.F., van den Briel, W., Musgrave, A., van den Ende, H. (1980) Sex specific glycoproteins in Chlamydomonas flagella. An immunological study. Arch. Microbiol. 126, 77–81
Lens, P.F., Olofsen, F., Nederbracht, A., Musgrave, A., van den Ende, H. (1982) An antiserum against a glycoprotein functional in flagellar adhesion between Chlamydomonas eurgametos gametes. Arch. Microbiol. 131, 241–246
Mesland, D.A.M. (1976) Mating in Chlamydomonas eugametos. A scanning electron microscopical study. Arch. Microbiol. 109, 31–35
Mesland, D.A.M., van den Ende, H. (1978) The role of flagellar adhesion in sexual activation of Chlamydomonas eugametos. Protoplasma 98, 115–129
Musgrave, A., van Eijk, E., te Welscher, R., Broekman, R., Lens, P., Homan, W., van den Ende, H. (1981) Sexual agglutination factor from Chlamydomonas eugametos. Planta 153, 362–369
Remillard, S.P., Witman, G.B. (1982) Synthesis, transport and utilization of specific flagellar proteins during flagellar regeneration in Chlamydomonas. J. Cell Biol. 93, 615–631
Roberts, K., Gurney-Smith, M., Hills, G.J. (1972) Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardtii. J. Ultrastruct. Res. 40, 599–613
Rogalski, A.A., Bouck, G.B. (1982) Flagellar surface antigens in Euglena: immunological evidence for an external glycoprotein pool and its transfer to the regenerating flagellum. J. Cell Biol. 93, 758–766
Rosenbaum, J.L., Moulders, J.E., Ringo, D.L. (1969) Flagellar elongation and shortening in Chlamydomonas. J. Cell Biol. 41, 600–619
Schlösser, U.G., Sachs, H., Robinson, D.G. (1976) Isolation of protoplasts by means of a “species-specific” autolysine in Chlamydomonas. Protoplasma 88, 51–64
Snell, W.J., Moore, W.S. (1980) Aggregation dependent turnover of flagellar adhesion molecules in Chlamydomonas gametes. J. Cell Biol. 84, 203–210
Van den Ende, H. (1981) Sexual interactions in the green alga Chlamydomonas eugametos. In: Sexual interactions in eukaryotic microbes, pp. 297–318, O'Day, D., Horgen, P.A., eds.) Academic Press, London New York San Francisco
Weeks, D.P., Collis, P.S. (1976) Induction of microtubule protein synthesis in Chlamydomonas reinhardtii during flagellar regeneration. Cell 9, 25–27
Weiss, R.L., Goodenough, D.A., Goodenough, U.W. (1977) Membrane particle arrays associated with the basal body and with contractile vacuole secretion in Chlamydomonas. J. Cell Biol. 72, 133–143
Wiese, L. (1965) On sexual agglutination and mating type substances (gamones) in isogamous heterothallic Chlamydomonads. J. Phycol. 1, 46–54
Wiese, L., Wiese, W. (1978) Sex cell contact in Chlamydomonas, a model for cell recognition. Symp. Soc. Exp. Biol. 32, 83–104
Witman, G.B.K., Carlson, J., Berliner, J., Rosenbaum, J.L. (1972) Chlamydomonas flagella. I. Isolation and electrophoresic analysis of microtubules, matrix, membranes and mastigonemes. J. Cell Biol. 54, 507–539
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Pijst, H.L.A., Zilver, R.J., Musgrave, A. et al. Agglutination factor in the cell body of Chlamydomonas eugametos . Planta 158, 403–409 (1983). https://doi.org/10.1007/BF00397732
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DOI: https://doi.org/10.1007/BF00397732