Abstract
Extracts prepared from dry pea (Pisum sativum, L; cv oberon) primary axes translate efficiently their endogenous messengers in an in vitro protein synthesizing system. The native long-lived messengers are biologically fully active and direct the synthesis of a whole range of polypeptides with MW ranging up to 130,000. About 0.5% of the total in vitro synthesized polypeptides are recovered in the immunoprecipitate obtained with pea lectin antiserum. Since about one-fourth of the radioactivity in the immunoprecipitate comigrates with authentic pea lectin it is concluded that about 0.1% of the long-lived messengers code for the lectin.
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Abbreviations
- mRNA:
-
messenger RNA
- mRNP:
-
messenger ribonucleoprotein
- SDS:
-
sodium dodecyl sulphate
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-ethanesulphonic acid
- S.A:
-
specific activity
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Peumans, W.J., Delaey, B.M., Manickam, A. et al. Efficient translation of long-lived messengers in extracts from dry pea primary axes. Planta 150, 286–290 (1980). https://doi.org/10.1007/BF00384657
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DOI: https://doi.org/10.1007/BF00384657