Abstract
A specific protein—an inhibitor of Colletotrichum lindemuthianum protease—was isolated from kidney bean seeds in a homogeneous form. The purification procedure included gel filtration, isoelectric focusing and affinity chromatography on trypsin-Sepharose column. The latter was introduced to separate the fungal protease inhibitor from closely related trypsin and chymotrypsin inhibitors present in kidney bean seeds.
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Mosolov, V.V., Loginova, M.D., Malova, E.L. et al. A specific inhibitor of Colletotrichum lindemuthianum protease from kidney bean (Phaseolus vulgaris) seeds. Planta 144, 265–269 (1979). https://doi.org/10.1007/BF00388768
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DOI: https://doi.org/10.1007/BF00388768