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Lokalisation der Mannitbiosynthese in der marinen Braunalge Fucus serratus

Localization of mannitol biosynthesis in the marine brown alga Fucus serratus

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Summary

Tips of fronds of Fucus serratus L. were exposed to H14CO3 in the light for periods of 10, 30, 60, and 180s, fixed in petrol ether at-70° C, and subsequently lyophilized. Pheoplasts (=chloroplasts) were isolated using the nonaqueous technique of Thalacker et al. (1959). After extraction and chromatography percentage 14C activity and distribution of individual photoassimilates between pheoplasts and other compartments of assimilating cells were analyzed. Eighty percent of [14C]-phosphate esters were found within the pheoplasts after 10s 14C-assimilation, whereas only 25% were found there after 30s. After 10s [14C] mannitol is almost totally localized within the plastids, but after 180s the major part has been localized outside the pheoplasts. On the basis of these data the pheoplasts are regarded to be the only sites of primary mannitol biosynthesis during photosynthesis in Fucus.

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Literatur

  • Arnon, D. J.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949).

    Google Scholar 

  • Bassham, J. A., Kirk, M., Jensen, R. G.: Photosynthesis by isolated chloroplasts. I. Diffusion of labelled photosynthetic intermediates between isolated chloroplasts and suspending medium. Biochim. biophys. Acta (Amst.) 153, 211–218 (1968).

    Google Scholar 

  • Bidwell, R. G. S., Craigie, J. S., Krotkov, G.: Photosynthesis and metabolism of marine algae. III. Distribution of photosynthetic carbon from 14CO2 in Fucus vesiculosus. Canad. J. Bot. 36, 581–590 (1958).

    Google Scholar 

  • Bird, J. F., Porter, H. K., Stocking, C. R.: Intracellular localization of enzymes associated with sucrose synthesis in leaves. Biochim. biophys. Acta (Amst.) 100, 366–375 (1965).

    Google Scholar 

  • Everson, R. G., Cockburn, W., Gibbs, M.: Sucrose as a product of photosynthesis in isolated spinach chloroplasts. Plant Physiol. 42, 840–844 (1967).

    Google Scholar 

  • Feige, B., Gimmler, H., Jeschke, W. D., Simonis, W.: Eine Methode zur dünnschichtchromatographischen Auftrennung von 14C-und 32P-markierten Stoffwechselprodukten. J. Chromatography 41, 80–90 (1969).

    Article  Google Scholar 

  • Havir, E. A., Gibbs, M.: Studies on the reductive pentose phosphate cycle in intact reconstituted chloroplast systems. J. biol. Chem. 238, 3183–3187 (1963).

    PubMed  Google Scholar 

  • Heber, U.: Transport of metabolites in photosynthesis. In: Biochemistry of chloroplasts, vol. II, p. 71–78, Goodwin, T. W., ed. London: Academic Press 1967.

    Google Scholar 

  • Heber, U., Krause, G. H.: Transfer of carbon, phosphate energy and reducing equivalents across the chloroplast envelope. In: Photosynthesis and photorespiration, p. 218–225, Hatch, M. D., Osmond, C. B., Slatyer, R. O., eds. New York: Wiley 1971.

    Google Scholar 

  • Heber, U., Santarius, K. A.: Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforsch. 25b, 718–727 (1970).

    Google Scholar 

  • Heber, U., Willenbrink, J.: Sites of synthesis and transport of photosynthetic products within the leaf cell. Biochim. biophys. Acta (Amst.) 82, 313–324 (1964).

    Google Scholar 

  • Jensen, R. G., Bassham, J. A.: Photosynthesis by isolated chloroplasts Proc. nat. Acad. Sci. (Wash.) 56, 1095–1101 (1966).

    Google Scholar 

  • Kremer, B. P., Willenbrink, J.: CO2-Fixierung und Stofftransport in benthischen marinen Algen. I. Zur Kinetik der 14CO2-Assimilation bei Laminaria saccharina. Planta (Berl.) 103, 55–64 (1972).

    Google Scholar 

  • Sachs, J.: Über den Einfluß des Lichtes auf die Bildung des Amylums in den Chlorophyllkörnern. Bot. Z. 20, 365–373 (1862).

    Google Scholar 

  • Stocking, C. R., Williams, R., Ongun, A.: Intracellular distribution of the early products of photosynthesis. Biochem. biophys. Res. Commun. 10, 416–421 (1963).

    PubMed  Google Scholar 

  • Thalacker, R., Behrens, M.: Über den Reinheitsgrad der in einem nichtwäßrigen spezifischen Gewichtsgradienten gewonnenen Chloroplasten. Z. Naturforsch. 14b, 443–446 (1959).

    Google Scholar 

  • Winkenbach, F., Parthasarathy, M. V., Bidwell, R. G. S.: Sites of photosynthetic metabolism in cells and chloroplast preparations of Acetabularia mediterranea. Canad. J. Bot. 50, 1367–1375 (1972).

    Google Scholar 

  • Yamaguchi, T., Ikawa, T., Nisizawa, K.: Incorporation of radioactive carbon from H14CO3 into sugar constituents by a brown alga, Eisenia bicyclis, during photosynthesis and its fate in the dark. Plant Cell Physiol. 7, 217–229 (1966).

    Google Scholar 

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Authors and Affiliations

  1. Botanisches Institut der Universität zu Köln, Gyrhofstraße 15, D-5000, Köln 41, Federal Republic of Germany

    Johannes Willenbrink & Bruno P. Kremer

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  1. Johannes Willenbrink
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  2. Bruno P. Kremer
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Willenbrink, J., Kremer, B.P. Lokalisation der Mannitbiosynthese in der marinen Braunalge Fucus serratus . Planta 113, 173–178 (1973). https://doi.org/10.1007/BF00388201

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  • DOI: https://doi.org/10.1007/BF00388201

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