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Nachweis und Lokalisation von Tryptophan-Synthetase in Samen von Juglans regia L.

Identification and localisation of tryptophan synthetase in seeds of Juglans regia L.

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Summary

Cell-free extracts from seeds of Juglans regia synthesize tryptophan from L-serine and indole. Tryptophan synthetase has maximal activity in the range between pH 7 and 8. The enzyme is associated with a particulate fraction (density 1,210 g/ml) which is separated from the mitochondria (density 1,191 g/ml) after isopycnic density centrifugation on a continuous sucrose gradient.

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Authors and Affiliations

  1. Botanisches Institut der Universität zu Köln, Gyrhofstraße 15, D-5000, Köln 41, Bundesrepublik Deutschland

    Wolfgang Große & Helga Witzsche

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  1. Wolfgang Große
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  2. Helga Witzsche
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Große, W., Witzsche, H. Nachweis und Lokalisation von Tryptophan-Synthetase in Samen von Juglans regia L.. Planta 111, 65–71 (1973). https://doi.org/10.1007/BF00386736

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  • DOI: https://doi.org/10.1007/BF00386736

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