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Production of an antiserum against cyclic nucleotide phosphodiesterase and its use for the immunocytochemical demonstration of this enzyme in rat cerebellum

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Summary

A procedure for the separation of cyclic AMP phosphodiesterase from a commercially available preparation and for raising antibodies against this enzyme in rabbits is described. An antiserum thus obtained was used for the immunocytochemical detection of cyclic nucleotide phosphodiesterase in rat cerebellum. The molecular layer, the granular layer and the cerebellar white matter exhibited different degrees of immunoreactivity. Only a few cell bodies (possibly glial cells) were stained. Most of the antigenic sites were present in the neuropil of the molecular layer and around Purkinje cells. Cerebellar glomeruli, sites of synaptic interactions between mossy fibres, Golgi cells and granule cells, were also stained by this antiserum. Control reactions using preimmune serum were consistently negative.

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Authors and Affiliations

  1. Institute for Neurobiology and Brain Research, Acadamy of Sciences, Leipziger Strasse 44, DDR-3090, Magdeburg, German Democratic Republic

    G. Poeggel

  2. Section of Biosciences, Karl Marx University Leipzig, Talstrasse 33, DDR-7010, Leipzig, German Democratic Republic

    H. Luppa, W. Ludwig & P. Borneleit

Authors
  1. G. Poeggel
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  2. H. Luppa
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  3. W. Ludwig
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  4. P. Borneleit
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Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday.

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Poeggel, G., Luppa, H., Ludwig, W. et al. Production of an antiserum against cyclic nucleotide phosphodiesterase and its use for the immunocytochemical demonstration of this enzyme in rat cerebellum. Histochemistry 88, 513–518 (1988). https://doi.org/10.1007/BF00570318

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  • DOI: https://doi.org/10.1007/BF00570318

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