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Quantitation of sulfhydryl groups on erythrocytes in polycythemia vera

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Summary

The number of sulfhydryl groups on the surface of intact erythrocytes from patients with polycythemia vera is significantly less (34%) than those from control subjects. Sulfhydryl groups were measured indirectly by reversibly blocking-SH groups with the thiol reagent, 6,6′-dithiodinicotinic acid, which forms stable mixed disulfides on the surface of erythrocytes. Glutathione was used to break the disulfides and release thione into the supernatant. Thione was then quantitated spectrophotometrically.

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Authors and Affiliations

  1. Department of Anatomy, Quillen-Dishner College of Medicine, East Tennessee State University, 37614, Johnson City, Tennessee, USA

    M. W. Hougland & J. M. Luethke

Authors
  1. M. W. Hougland
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  2. J. M. Luethke
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Supported by NIH Biomedical Research Development Grant 1-S08-RR-09171-01

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Hougland, M.W., Luethke, J.M. Quantitation of sulfhydryl groups on erythrocytes in polycythemia vera. Histochemistry 76, 57–60 (1982). https://doi.org/10.1007/BF00493285

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  • DOI: https://doi.org/10.1007/BF00493285

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