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Fluorescent histochemical demonstration of cathepsin B in the rat yolk sac

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Summary

Fluorescence demonstration of cathepsin B (E.C. 3.4.22.1) activity was performed in the yolk sac of rats near term (18th day of gestation). The enzyme demonstration was performed on freeze-dried and celloidin mounted yolk-sac sections using different substituted β-naphthylamide derivatives as substrates and nitrosalicylaldehyde as coupling agent. The discrete reaction products are localized preferentially in the apical part of the visceral yolk-sac epithelium. There is little doubt that cathepsin B is contained here in the well developed lysosomal apparatus of the yolk-sac epithelium.

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Authors and Affiliations

  1. Institute of Anatomy, University of Würzburg, Koellikerstrasse 6, D-8700, Würzburg, Federal Republic of Germany

    P. Kugler

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  1. P. Kugler
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Supported by the Deutsche Forschungsgemeinschaft (SFB 105)

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Kugler, P. Fluorescent histochemical demonstration of cathepsin B in the rat yolk sac. Histochemistry 75, 215–218 (1982). https://doi.org/10.1007/BF00496013

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  • DOI: https://doi.org/10.1007/BF00496013

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