Summary
Aminopeptidase A (E.C.3.4.11.7; APA) can be demonstrated histochemically in the rat and mouse kidney by light microscopy (simultaneous azo coupling with α-Glu-MNA as substrate and high-purity FBB as coupling agent) mainly in the brush borders, glomeruli and portions of the juxtaglomerular apparatus. Sex and species differences are found with regard to enzyme activity and localization. The relation of aminopeptidase A to angiotensinase A was established by inhibition experiments with angiotensin II and III. The following significant differences exist with respect to other aminopeptidases (aminopeptidase M and γ-glutamyl transferase), which were also demonstrated: APM shows no dependence on calcium ions; APM and γ-GT are not demonstrable in the glomerulus or juxtaglomerular apparatus.
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References
Beaufils M, Sraer J, Lepreux C, Ardaillon R (1976) Angiotensin II binding to renal glomeruli from sodium-loaded and sodium-depleted rats. Am J Physiol 230:1187–1193
Blantz RC (1980) The glomerulus, passive filter or regulatory organ? Klin Wochenschr 58:957–964
Glenner GG, Folk JE (1961) Glutamyl peptidases in rat and guinea-pig kidney slices. Nature (London) 192:338–340
Glenner GG, Mc Millan PHJ, Folk LF (1962a) A mammalian peptidase specific for the hydrolysis of N-terminal α-l-glutamyl and aspartyl residues. Nature (London) 194:867
Glenner GG, Folk GE, Mc Millan PHJ (1962b) Histochemical demonstration of a gamma-glutamyl transpeptidase-like activity. J Histochem Cytochem 10:481–489
Gorgas K (1978) Struktur und Innervation des juxtaglomerulären Apparates der Ratte. Adv Anat Embryol 54/2
Hess R (1965) Arylamidase activity related to angiotensinase. Biochim Biophys Acta 99:316–324
Hierholzer K (1977) Das Renin-Angiotensin-Aldosteronsystem. In: Hierholzer K, Neubert D, Quabbe HJ Endokrinologie III. Physiologie des Menschen. Vol 20. Hrsg.: Gauer OH, Kramer K, Jung R. Urban & Schwarzenberg, München Wien Baltimore
Johnson JA, Anderson RR (1980) The renin-angiotensin system. Advances in experimental medicine and biology, Vol 130. Plenum Press, New York London
Leary WP, Ledingham JG (1969) Removal of angiotensin by isolated perfused organs of the rat. Nature (London) 222:959–960
Lojda Z, Gossrau R, Schiebler TH (1979) Enzyme histochemistry. A laboratory manual. Springer, Berlin Heidelberg New York
Lojda Z, Gossrau R (1980) Study on aminopeptidase A. Histochemistry 67:267–290
Maunsbach AB (1966) Observations on the segmentation of the proximal tubule in the kidney. Comparison of results from phase contrast, fluorescence and electron microscopy. J Ultrastruct Res 16:239–258
Möllendorf W von (1930) Der Exkretionsapparat. In: Handbuch der mikroskopischen Anatomie des Menschen. Bd. VII/1. Springer, Berlin
Nagatsu J, Nagatsu T, Yamamoto T, Glenner GG, Mehl JW (1970) Purification of aminopeptidase A in human serum and degradation of angiotensin II by the purified enzyme. Biochim Biophys Acta 198:255–270
Peach JM, Levens NR (1980) Molecular approaches to the study of angiotensin receptors. In: Johnson JA, Anderson RR (eds) The renin-angiotensin system. Advances in experimental medicine and biology, Vol 130. Plenum Press, New York London
Rutenberg AM, Kim H, Fischbein JW, Hanker JS, Wasserkrug HL, Seligman AM (1969) γ-Glutamyl transpeptidase activity. J Histochem Cytochem 17:517–526
Srauer J, Band L, Cosyns JP, Verroust P, Nivez MP, Ardaillon R (1977) High affinity binding of 125J-angiotensin II to rat glomerular membranes. J Clin Invest 59:69–81
Thurau K (1973) Die Bedeutung des juxtaglomerulären Apparates für die Funktion des Nephrons. Verh Anat Ges 67:137–150
Wachsmuth ED (1968) Lokalisation von Aminopeptidase in Gewebeschnitten mit einer neuen Immunfluoreszenztechnik. Histochemie 14:282–296
Wachsmuth ED, Torhorst A (1974) Possible precursors of aminopeptidase and alkaline phosphatase in the proximal tubules of kidney and the crypts of small intestine of mice. Histochemistry 38:43–56
Wachsmuth ED, Donner P (1976) Conclusions about aminopeptidase in tissue sections from studies of amino acid naphthylamide hydrolysis. Histochemistry 47:271–283
Winckler J (1970a) Zum Einfrieren von Gewebe in stickstoff-gekühltem Propan. Histochemie 23:44–50
Winckler J (1970b) Kontrollierte Gefriertrocknung von Kryostatschnitten. Histochemie 22:234–240
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Kugler, P. Localization of aminopeptidase A (angiotensinase A) in the rat and mouse kidney. Histochemistry 72, 269–278 (1981). https://doi.org/10.1007/BF00517140
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DOI: https://doi.org/10.1007/BF00517140