Summary
The results presented in this paper show that collagen fibers can be clearly distinguished from reticular fibers using the picrosirius-polarization method. A morphologic and morphometric study of these two types of fibers with electron microscopy shows that reticular fibers are characterized by the smaller diameter of their fibrillar components and the higher content of interfibrillar material, resulting in a loose arrangement of the fibrils. The evidences presented suggest that the amorphous matrix in which fibrils are embedded is responsible for the silver impregnation of reticular fibers. Our results show that the matrix of reticular fibers is characteristically rich in heparitin sulfate, and that the glycosaminoglycans present show a high interaction with the fibrillar component of these fibers.
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Becker, U., Nowack, H., Gay, S., Timpl, R.: Production and specificity of antibodies against the aminoterminal region in type III collagen. Immunology, 31, 57–65 (1976)
Bloom, W., Fawcett, D.W.: A textbook of histology. Philadelphia: Saunders and Co. 1975
Dietrich, C.P., Dietrich, S.M.C.: Electrophoretic behaviour of acidic mucopolysaccharides in diamine buffers. Anal. Biochem. 70, 645–647 (1976)
Fernando, N.V.P., van Erkel, G.A., Movat, H.Z.: The fine structure of connective tissues. IV. The intercellular elements. Exp. Mol. Pathol. 3, 529–545 (1964)
Foot, N.C.: Chemical contrasts between collagenous and reticular connective tissue. Am. J. Pathol. 4, 525–544 (1928)
Gamble, H.J., Eames, R.A.: An electron microscope study of the connective tissues of the human peripheral nerve. J. Anat. 98, 655–663 (1964)
Glegg, R.E., Eidinger, D., Leblond, C.P.: Some carbohydrate components of reticular fibers. Science 118, 614–616 (1953)
Ishii, T.: Das Silberbild der Retikulinfasern des Lymphknotens (mit besonderer Berücksichtigung des Übergangs in die kollagenen Fasern). Verh. Anat. Ges. 62, 353–359 (1967)
Junqueira, L.C.U., Cossermelli, W., Brentani, R.: Differential staining of collagens type I, II and III by Sirius Red and polarization microscopy. Arch. Histol. Jpn. 41, 267–274 (1978)
Junqueira, L.C.U., Bignolas, G., Brentani, R.: Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem. J. 11, 447–455 (1979a)
Junqueira, L.C.U., Bignolas, G., Mourão, P.A.S., Bonetti, S.S.: Quantitation of collagen-proteoglycans interaction in tissue sections. Connect. Tissue Res. (1979b; in press)
Junqueira, L.C.U., Montes, G.S., Krisztán, R.M.: The collagen of the vertebrate peripheral nervous system. Cell Tissue Res. (1979c; in press)
Kupffer, C.W.: Über Sternzellen der Leber: Briefliche Mitteilung an Prof. Waldeyer. Arch. Mikrosk. Anat. 12, 353–358 (1876)
Luna, L.G. (ed.): Manual of histologic staining methods of the Armed Forces Institute of Pathology. New York: McGraw-Hill 1968
Mall, F.: Reticulated and yellow elastic tissues. Anat. Anz. 3, 397–401 (1888)
Mallory, F.B., Parker, F.: Reticulum. Am. J. Pathol. 3, 515–525 (1927)
Maresch, R.: Über Gitterfasern der Leber und die Verwendbarkeit der Methode Bielschowskys zur Darstellung feinster Bindegewebsfibrillen. Zentralbl. Allg. Pathol. Pathol. Anat. 16, 641–649 (1905)
Melcher, A.H.: Gingival reticulin: Identification and role in histogenesis of collagen fibers. J. Dent. Res. 45, 426–439 (1966)
Mourão, P.A.S., Rozenfeld, S., Laredo, J., Dietrich, C.P.: The distribution of chondroitin sulfates in articular and growth cartilages of human bones. Biochim. Biophys. Acta 428, 19–26 (1976)
Naoumenko, J., Feigin, I.: A simple silver solution for staining reticulin. Stain Technol. 49, 153–155 (1974)
Peters, A., Palay, S., Webster, H.: The fine structure of the nervous system. The neurons and supporting cells. Philadelphia: Saunders and Co. 1976
Plenk, H.: Über argyrophile Fasern (Gitterfasern) und ihre Bildungszellen. Ergebn. Anat. Entwicklungsgesch. 27, 302–412 (1927)
Shepard, N., Mitchell, N.: The localization of articular cartilage proteoglycan by electron microscopy. Anat. Rec. 187, 463–476 (1977)
Snodgrass, M.J.: Ultrastructural distinction between reticular and collagenous fibers with an ammoniacal silver stain. Anat. Rec. 187, 191–206 (1977)
Thomas, P.K.: The connective tissue of peripheral nerve: an electron microscope study. J. Anat. 97, 35–44 (1963)
Thomas, P.K., Jones, D.G.: The cellular response to nerve injury. 2. Regeneration of the perineurium after nerve section. J. Anat. 101, 45–55 (1967)
Toledo, O.M.S., Dietrich, C.P.: Tissue specific distribution of sulfated mucopolysaccharides in mammals. Biochim. Biophys. Acta 498, 114–122 (1977)
Weibel, E.R.: Principles and methods for the morphometric study of the lung and other organs. Lab. Invest. 12, 131–155 (1963)
Windrum, G.M., Kent, P.W., Eastoe, J.E.: The constitution of human renal reticulin. Br. J. Exp. Pathol. 36, 49–59 (1955)
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Montes, G.S., Krisztán, R.M., Shigihara, K.M. et al. Histochemical and morphological characterization of reticular fibers. Histochemistry 65, 131–141 (1980). https://doi.org/10.1007/BF00493161
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DOI: https://doi.org/10.1007/BF00493161