Summary
Rats treated with triparanol (MER-29) develop numerous membranous inclusions-“myeloid” bodies in the cytoplasm of liver cells. The “myeloid” bodies did not show cytochemically demonstrable acid phosphatase. Instead diffuse activity was observed throughout the cytoplasm. Biochemically, acid phosphatase was found in the liver lysosomal fraction obtained from triparanol treated rats. This fraction, however, did not show the structure-linked latency of acid phosphatase which is characteristic of normal lysosomes. It is suggested that “myeloid” bodies are lysosomes with altered membranes.
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References
Abraham, R., Hendry, R.: Effects of chronic chloroquine treatment on lysosomes of rat liver cells. Exp. mol. Path. 12, 148–159 (1970).
Allen, J.M., Gockerman, J.: Electrophoretic separation of multiple forms of associated acid phosphatase. Ann. N. Y. Acad. Sci. 121, 616–633 (1964).
Avigan, J., Steinberg, D., Thompson, M.J., Mosetting, E.: The mechanism of action of MER-29. Progr. cardiovasc. Dis. 2, 525–530 (1960).
Barka, T., Anderson, P.J.: Histochemical methods for acid phosphatase using hexazonium paraosanilin as coupler. J. Histochem. Cytochem. 10, 741–753 (1962).
Bitensky, L.: The demonstration of lysosomes by the controlled temperature freezing sectioning method. Quart. J. micr. Sci. 103, 205–209 (1962).
Blohm, T.R., Mackenzie, R.D.: Specific inhibition of cholesterol biosynthesis by a synthetic compound (MER-29). Arch. Biochem. Biophys. 85, 243–249 (1959).
De Duve, C.: The lysosome concept. In: Ciba Foundation Symposium on Lysosomes (ed. by A. V. S. Reuck, M.P. Cameron) Boston, Mass.: Little, Brown & Co. 1963.
Dieter, S.E., Scallen, T.J.: An ultrastructural and biochemical study of the effects of three inhibitors of cholesterol biosynthesis upon murine adrenal gland and testis.Histochemical evidence for a lysosomal response. J. Cell. Biol. 40, 44–60 (1969).
Gahan, P.B.: Histochemistry of lysosomes. Int. Rev. Cytol. 21, 1–63 (1967).
Goldfischer, S.: The cytochemical demonstration of lysosomal aryl sulfatase activity by light and electron microscopy. J. Histochem. Cytochem. 13, 520–523 (1965).
Hayashi, M., Nakajima, Y., Fishman, W.H.: The cytologic demonstration of β-glucuronidase employing naphtol AS-BI glucuronide and hexazonium paraosanilin, a preliminary report. J. Histochem. Cytochem. 12, 293–297 (1964).
Hruban, Z., Slesers, A., Hopkins, E.: Drug-induced and naturally occurring myeloid bodies. Lab. Invest. 27, 62–70 (1972).
Hruban, Z., Swift, H., Slesers, A.: Effect of triparanol and diethanolamine on the fine structure of hepatocytes and pancreatic acinar cells. Lab. Invest. 14, 1652–1672 (1965).
Rubright, W.C., Keller, O., Hruban, Z.: Ultrastructure changes produced by triparanol in Morris 5123 and Novikoff Hepatomas. Cancer Res. 27, 165–171 (1967).
Scallen, T.J., Condie, R.M., Schroepfer, G.J., Jr.: Inhibition by triparanol of cholesterol formation in the brain of newborn mouse. J. Neurochem. 9, 99–103 (1962).
Schutta, H.S., Neville, H.E.: Effects of cholesterol synthesis inhibitors on the nervous system. Lab. Invest. 19, 487–493 (1968).
Shinowara, G., Jones, L., Reinhart, H.: The estimation of serum inorganic phosphate and acid alkaline phosphatase activity. J. biol. Chem. 142, 921–929 (1942).
Sloat, B.F., Allen, J.M.: Soluble and membrane associated forms of acid phosphatase associated with lysosomal fraction of rat liver. Ann. N. Y. Acad. Sci. 175, 574–601 (1969).
Volk, T.L., Scarpelli, D.C.: Alterations of fine structure of the rat adrenal cortex after the administration of triparanol. Lab. Invest. 13, 1205–1214 (1964).
Yates, R.D.: Effect of triparanol on adrenocortical cells of the zona fasiculata of Syrian hamsters. Z. Zellforsch. 71, 41–52 (1966).
Yates, R.D., Arai, K., Rappaport, D.A.: Fine structure and chemical composition of opaque cytoplasmic bodies of triparanol-treated Syrian hamsters. Exp. Cell Res. 47, 459–478 (1967).
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Kalina, M., Lopez, M.L. & Bubis, J.J. Effect of triparanol (Mer 29) on rat liver lysosomes: cytochemical and biochemical study. Histochemie 36, 5–14 (1973). https://doi.org/10.1007/BF00310115
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DOI: https://doi.org/10.1007/BF00310115