Summary
The inhibition of rat small intestinal cytochrome c oxydase by in vivo administration of chloramphenicol and oxytetracycline has been demonstrated histochemically. Since the dwelling time of newly formed epithelial cells in the crypts of jejunum is 10–14 h, it was surprising to find inhibition of cytochrome c oxydase after 12 h treatment not only in the crypts but also in the villus. These experiments led to the conclusion that cytochrome c oxidase is continuously synthesized, and probably also degraded in the villous cells. Coupling of oxidative phosphorylation is still present after 48 h antibiotic treatment, when the effect on cytochrome c oxydase appeared to be maximal, as judged by the persistance of 2,4-dinitro-phenol-stimulated adenosinetriphosphatase. Finally, prolonged (⩾48 h) antibiotic treatment often led to retraction of connective tissue (which supports the villous epithelial cells), resulting in loosening and loss of cells from the tops of the villi.
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References
Burstone, M. S.: New histochemical techniques for the demonstration of tissue oxidase (cytochrome oxidase). J. Histochem. Cytochem. 7, 112–122 (1959).
Cairnie, A. B., Lamerton, L. F., Steel, G. G.: Cell proliferation studies in the intestinal epithelium of the rat. I. Determination of the kinetic parameters. Exp. Cell Res. 39, 528–538 (1965).
Firkin, F. C., Linnane, A. W.: Biogenesis of mitochondria. 8. The effect of chloramphenicol on regenerating rat liver. Exp. Cell Res. 55, 68–76 (1969).
Goldfisher, S., Essner, E., Novikoff, A. B.: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem. 12, 72–76 (1964).
Hülsmann, W. C., Iemhoff, W. G. J., Berg, J. W. O. v. d., Pijper, A. M. de: Unequal rates of development of mitochondrial enzymes in rat small intestinal epithelium. Biochim. biophys. Acta (Amst.) 215, 553–555 (1970).
Lemhoff, W. G. J., Berg, J. W. O. v. d., Pijper, A. M. de, Hülsmann, W. C.: Metabolie aspects of isolated cells from rat small intestinal epithelium. Biochim. biophys. Acta (Amst.) 215, 229–241 (1970).
Iemhoff, W. G. J., Hülsmann, W. C.: Development of mitochondrial enzyme activities in rat small intestinal epithelium. Europ. J. Biochem. 23, 429–434 (1971).
Jonge, H. R. de: In preparation (1972).
Jonge, H. R. de, Hülsmann, W. C.: Inhibition of mitochondrial protein synthesis in rat small intestinal epithelium. Submitted for publication (1972).
McMillan, P. J.: Differential demonstration of muscle and heart type lactic dehydrogenase of rat muscle and kidney. J. Histochem. Cytochem. 15, 21–31 (1967).
Meijer, A. E. F. H.: Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. I. Acid phosphatase. Histochemie 30, 31–39 (1972).
Sherman, F., Stewart, J. W.: Genetics and biosynthesis of cytochrome c. Ann. Rev. Genet. 5, 257–296 (1971).
Vries, H. de, Kroon, A. M.: On the effect of chloramphenicol and oxytetracycline on the biogenesis of mammalian mitochondria. Biochim. biophys. Acta (Amst.) 204, 531–541 (1970).
Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatase at a physiologic pH. Amer. J. clin. Path. 27, 13–23 (1957).
Webster, H. L., Harrison, D. D.: Enzymic activities during the transformation of crypt to columnar intestinal cells. Exp. Cell Res. 56, 245–253 (1969).
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de Pijper, A.M., Hülsmann, W.C. The influence of in vivo administered chloramphenicol and oxytetracycline on some mitochondrial enzymes of rat-small-intestinal epithelium: Histochemical data. Histochemie 33, 181–190 (1972). https://doi.org/10.1007/BF00305745
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DOI: https://doi.org/10.1007/BF00305745