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Metachromasia induced by inositol-hexa-phosphate with toluidine blue

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Summary

  1. 1.

    Metachromasia with toluidine blue could be induced by inositol hexaphosphate, the smallest chromotrope molecule so far reported.

  2. 2.

    The metachromatic compound, pink in color, and distinct from orthochromatic dye, could be separated by centrifugation. This complex could as well be decomposed by dil. HCl.

  3. 3.

    The stoichiometry of the metachromatic complex has been studied and the molar ratio of dye: phytate has been shown to vary with varying proportions of dye and phytate used in the reaction mixture and a maximum value of 10 could be obtained suggesting a steric hindrance for the attachment of dye to the phytate-molecule.

  4. 4.

    The divalent cations like, Ba++, Mg++, and Mn++ interfered with the formation of metachromatic complex, possibly competing with the dyes for the anionic sites of the phytate molecule.

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References

  • Agranoff, B. W., R. M. Bradley, and R. O. Brady: The enzymatic synthesis of inositol phosphatide. J. biol. Chem. 233, 1077–1083 (1958).

    Google Scholar 

  • Anderson, R. J.: The organic phosphoric acid of cotton seed meal, II Phytin. J. biol. Chem. 17, 141–150 (1914).

    Google Scholar 

  • Biswas, S., and B. B. Biswas: A novel enzyme system: Phytin phosphotransferase. Sci. and Cult. 31, 634–635 (1965).

    Google Scholar 

  • Dangschat, G., and H. O. L. Fischer: Acetonation and configuration of meso inositol. Naturwissenschaften 30, 146–147 (1942).

    Google Scholar 

  • Ehrlich, P.: Beiträge zur Kenntnis der Anilinfärbungen und ihrer Verwendung in der mikroskopischen Technik. Arch. mikr. Anat. 13, 263–267 (1877).

    Google Scholar 

  • Fogg, D. N., and N. T. Wilkinson: The colorimetric determination of phosphorus. Analyst 83, 406–414 (1958).

    Google Scholar 

  • Harris, A. F., and A. E. Sobel: Calcification XX. The cationic repression of metachromasy. Arch. Biochem. 74, 345–356 (1958).

    Google Scholar 

  • Kelley, J. W.: In: Heilbrunn, L. B., and F. Webber, Protoplasmatologia: Handbuch der Protoplasmaforschung, Vol. 2, Sect. D-2, p. 1. Wien: Springer 1956.

    Google Scholar 

  • Levine, A., and M. Schubert: Metachromatic effects of anionic polysaccharides and detergents. J. Amer. chem. Soc. 74, 5702–5706 (1952).

    Google Scholar 

  • McIntosh, F. C.: A colorimetric method for the standardization of heparin preparation. Biochem. J. 35, 776–782 (1941).

    Google Scholar 

  • McKay, R. B., and P. J. Hillson: Metachromatic behaviour of dyes in solvents of low dielectric constant. Trans. Faraday Soc. 62, 1439–1446 (1966).

    Google Scholar 

  • Michaelis, L., and S. Granick: Metachromasy of basic dyestuffs. J. Amer. chem. Soc. 67, 1212–1219 (1945).

    Google Scholar 

  • Mukherjee, P., and K. Mysels: A re-evaluation of the spectral change method of determining critical micelle concentration. J. Amer. chem. Soc. 77, 2937–2943 (1955).

    Google Scholar 

  • Neuberg, C.: Biochem. Z. 9, 557 (1908). Cit. by D. J. Cosgrove. In: „Chemistry and biochemistry of inositol polyphosphates. Rev. pure appl. Chem. 16, 209–224 (1966).

    Google Scholar 

  • Pal, M. K., and S. Basu: Polyelectrolytes chromotropes in metachromacy. Makromol. Chem. 27, 69–79 (1958).

    Google Scholar 

  • —, and M. Schubert: Ultracentrifugal separation of the metachromatic compound of methylene blue and chondroitin sulfate. J. physic. Chem. 65, 872–877 (1961a).

    Google Scholar 

  • —: Specific adsorption of metachromatic compounds of chondroitin sulfate by insoluble calcium salts. J. Histochem. Cytochem. 9, 673–680 (1961 b).

    Google Scholar 

  • —: Measurement of the stability of metachromatic compounds. J. Amer. chem. Soc. 84, 4384–4393 (1962).

    Google Scholar 

  • Rabinowitch, E., and L. F. Epstein: Polymerization of dyestuffs in solution thionine and methylene blue. J. Amer. chem. Soc. 63, 69–78 (1941).

    Google Scholar 

  • Schubert, M., and A. Levine: A conductometric study of the interaction of anionic mucopolysaccharides and cationic dyes. J. Amer. chem. Soc. 75, 5842–5846 (1953).

    Google Scholar 

  • Stone, A. L., and D. F. Bradley: Aggregation of cationic dyes on acid polysaccharides. 1. Spectrophotometric titration with acridine orange and other metachromatic dyes. Biochim. biophys. Acta (Amst.) 148, 172–192 (1967).

    Google Scholar 

  • Zanker, V.: The proof of definite reversible association of acridine orange by absorption and fluorescence measurements in aqueous solution. Z. physik. Chem. 199, 225–258 (1952).

    Google Scholar 

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Authors and Affiliations

  1. Bose Institute, Calcutta

    N. C. Mandal & B. B. Biswas

  2. Department of Chemistry, Faculty of Science, University of Kalyani, West Bengal, India

    M. K. Pal

Authors
  1. N. C. Mandal
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  2. B. B. Biswas
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  3. M. K. Pal
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Mandal, N.C., Biswas, B.B. & Pal, M.K. Metachromasia induced by inositol-hexa-phosphate with toluidine blue. Histochemie 18, 202–209 (1969). https://doi.org/10.1007/BF00306167

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  • DOI: https://doi.org/10.1007/BF00306167

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