Abstract
The structure of hen erythrocyte chromatin fibers was studied with the electron microscope. Chromatin fiber fragments with a length of about 5,000 Å and an average diameter of 320 Å are composed of 13 globular subunits (superbeads) which contain different numbers of nucleosomes. Their number average corresponds to 17 nucleosomes. — The interaction of lysine-rich histones with nucleosome chains was investigated by reconstitution experiments and was found to be semi-cooperative.
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Felsenfeld, G.: Chromatin-Review Article. Nature (Lond.) 271, 115–122 (1978)
Finch, J.T., Klug, A.: Solenoidal model for superstructure in chromatin. Proc. nat. Acad. Sci. (Wash.) 73, 1897–1901
Finch, J.T., Lutter, L.C., Rhodes, D., Brown, R.S., Rushton, B., Levitt, M., Klug, A.: Structure of nucleosome core particles of chromatin. Nature (Lond.) 269, 29–36 (1977)
Hozier, J.C., Kaus, R.: Subunit structure of chromosomes in mitotic nuclei of Physarum polycephalum: Chromosoma (Berl.) 57, 95–102 (1976)
Hozier, J., Renz, M., Nehls, P.: The chromosomes fiber: evidence for an ordered superstructure of nucleosomes. Chromosoma (Berl.) 62, 301–317 (1977)
Ilyin, Y.V., Varshavsky, A.Y., Mickelsaar, U.N., Georgiev, G.P.: Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA. Europ. J. Biochem. 22, 235–245 (1971)
Jones, E.W., Diggle, J.H.: A Method for the large scale preparation of the avian erythrocyte specific histone F2C. Europ. J. Biochem. 11, 495–498 (1969)
Kiryanov, G.I., Manamshjan, T.A., Polyakov, V.Yu., Fais, D., Chentsov, Ju.S.: Levels of granular organization of chromatin fibers. FEBS Letters 67, 323–327 (1976)
Kornberg, R.D.: Structure of chromatin. Ann. Rev. Biochem. 46, 931–954 (1977)
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227, 680–685 (1970)
Müller, U., Zentgraf, H., Eicken, I., Keller, W.: Higher-order structure of Simian virus 40 chromatin. Science 201, 406–415 (1978)
Renz, M.: Heterogeneity of the chromosome fiber. Nucleic Acids Res. 6, 2761 (1979)
Renz, N., Nehls, P., Hozier, J.: Involvement of histone H1 in the organization of the chromosome fiber. Proc. nat. Acad. Sci. (Wash.) 74, 1879–1883 (1977)
Ris, H., Korenberg, J.: Chromosome structure and levels of chromosome organization. Cell Biol. 2, 267–361 (1979)
Schaller, H., Nüsslein, Ch., Bonhoeffer, F.B., Kurz, Ch., Nietzschmann, I.: Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns. Europ. J. Biochem. 26, 474–481 (1972)
Scheer, U., Zentgraf, H.: Organization of rDNA circles in Dytiscus oocytes. Chromosoma (Berl.) 69, 243–254 (1978)
Suau, P., Bradbury, E.M., Baldwin, J.P.: Higher-order structures of chromatin in solution. Europ. J. Biochem. 97, 593–602 (1979)
Thomas, J.O.: Chromatin structure. Int. Rev. Biochem. 17, 181–232 (1978)
Weintraub, H.: The nucleosome repeat length increases during erythropoiesis in the chick. Nucleic Acids Res. 5, 1179–1188 (1978)
Worcel, A., Benyajati, C.: Higher order coiling of DNA in chromatin. Cell 12, 83–100 (1977)
Zentgraf, H., Falk, H., Franke, W.W.: Nuclear membranes and plasma membranes from hen erythrocytes. IV. Characterization of nuclear membrane attached DNA. Cytobiol. 11, 10–21 (1975)
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Meyer, G.F., Renz, M. Native and reconstituted chromosome fiber fragments. Chromosoma 75, 177–184 (1979). https://doi.org/10.1007/BF00292206
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DOI: https://doi.org/10.1007/BF00292206