Abstract
Fluorescence in situ hybridization using as probes three biotinylated or digoxigenin-labeled chicken W chromosome-specific repeating DNA units (0.7 and 1.1 kb XhoI family and 1.2 kb EcoRI family units) suggested that a large fraction of one arm of the W chromosome was occupied by the EcoRI family sequences and that pericentromeric regions were widely occupied by the XhoI family sequences. A minor fraction of the EcoRI family was also present in a narrow region in the proximal half of the other arm. There was a region in the distal half of the latter arm where sequences from neither family hybridized. Evolutionary aspects of the presence of different domains occupied by different repetitive families and the significance of the unhybridized distal region are discussed.
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References
Charlesworth C (1991) The evolution of sex chromosomes. Science 251: 1030–1033
Dover GA (1987) DNA turnover and molecular clock. J Mol Evol 26: 47–58
Kaelbling M, Fechheimer NS (1983) Synaptonemal complexes and the chromosome complement of domestic fowl, Gallus domesticus. Cytogenet Cell Genet 35: 87–92
Kodama H, Saitoh H, Tone M, Kuhara S, Sakaki Y, Mizuno S (1987) Nucleotide sequences and unusual electrophoretic behavior of the W chromosome-specific repeating DNA units of the domestic fowl, Gallus gallus domesticus. Chromosoma 96: 18–25
Mayr B, Auer H (1988) A method to induce Giemsa staining resistance of avian W chromosomes: detection of an additional W chromosome in a turkey. Genome 30: 395–398
Rahn MI, Solari AJ (1986) Recombination nodules in the oocytes of the chicken, Gallus domesticus. Cytogenet Cell Genet 43: 187–193
Saitoh Y, Saitoh H, Ohtomo K, Mizuno S (1991) Occupancy of the majority of DNA in the chicken W chromosome by bentrepetitive sequences. Chromosoma 101: 32–40
Simmler M-C, Rouyer F, Vergnaud G, Nystrom-Lahti M, Ngo KY, de laChapelle A, Weissenbach J (1985) Pseudoautosomal DNA sequences in the pairing region of the human sex chromosomes. Nature 317: 692–697
Singh PB, Miller JR, Pearce J, Kothary R, Burton RD, Paro R, James TC, Gaunt SJ (1991) A sequence motif found in a Drosophila heterochromatin protein is conserved in animals and plants. Nucleic Acids Res 19: 789–794
Solari AJ (1977) Ultrastructure of the synaptic autosomes and the ZW bivalent in chicken oocytes. Chromosoma 64: 155–165
Solari AJ, Fechheimer NS, Bitgood JJ (1988) Pairing of ZW genosomes and the localized recombination nodule in two Z-autosome translocations in Gallus domesticus. Cytogenet Cell Genet 48: 130–136
Solovei IV, Khutinayeva MA, Chelysheva LA, Rodionov AV, Gaginskaya ER (1990) Lamprush sex bivalent of the birds: Identification and structure. Citologiya 32: 816–823 (In Russian with English abstract)
Stefos K, Arrighi FE (1974) Repetitive DNA of Gallus domesticus and its cytological locations. Exp Cell Res 83: 9–14
Takagi N, Sasaki M (1974) A phylogenetic study of bird karyotypes. Chromosoma 46: 91–120
Tone M, Nakano N, Takao E, Narisawa S, Mizuno S (1982) Demonstration of W chromosome-specific repetitive DNA sequences in the domestic fows, Gallus g. domesticus. Chromosoma 86: 551–569
Tone M, Sakaki Y, Hashiguchi T, Mizuno S (1984) Genus specificity and extensive methylation of the W chromosome-specific repetitive DNA sequences from the domestic fowl, Gallus gallus domesticus. Chromosoma 89: 228–237
Trask B, Pinkel D (1990) Fluorescence in situ hybridization with DNA probes. Meth Cell Biol 33: 383–400
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by H.C. Macgregor
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Saitoh, Y., Mizuno, S. Distribution of XhoI and EcoRI family repetitive DNA sequences into separate domains in the chicken W chromosome. Chromosoma 101, 474–477 (1992). https://doi.org/10.1007/BF00352469
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DOI: https://doi.org/10.1007/BF00352469