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Comparative mapping of a gorilla-derived alpha satellite DNA clone on great ape and human chromosomes

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Abstract

We have isolated an alpha satellite DNA clone, pG3.9, from gorilla DNA. Fluorescence in situ hybridization on banded chromosomes under high stringency conditions revealed that pG3.9 identifies homologous sequences at the centromeric region of ten gorilla chromosomes, and, with few exceptions, also recognizes the homologous chromosomes in human. A pG3.9-like alphoid DNA is present on a larger number of orangutan chromosomes, but, in contrast, is present on only tow chromosomes in the chimpanzee. These results show that the chromosomal subsets of related alpha satellite DNA sequences may undergo different patterns of evolution.

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Author notes

  1. Antonio Baldini

    Present address: Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK

Authors and Affiliations

  1. Department of Human Genetics, Yale University School of Medicine, 06510, New Haven, CT, USA

    Antonio Baldini & David C. Ward

  2. Porto Conte Research and Training Laboratories, CNR Institute of Molecular Genetics, 1-07100, Sassari, Italy

    Antonio Baldini

  3. Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI, USA

    Dorothy A. Miller, Viji Shridhar & Orlando J. Miller

  4. Center for Molecular Biology, Wayne State University School of Medicine, Detroit, MI, USA

    Dorothy A. Miller, Viji Shridhar & Orlando J. Miller

  5. Institute of Genetics, University of Bari, I-Bari, Italy

    Mariano Rocchi

Authors
  1. Antonio Baldini
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  2. Dorothy A. Miller
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  3. Viji Shridhar
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  4. Mariano Rocchi
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  5. Orlando J. Miller
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  6. David C. Ward
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by J.B. Rattner

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Baldini, A., Miller, D.A., Shridhar, V. et al. Comparative mapping of a gorilla-derived alpha satellite DNA clone on great ape and human chromosomes. Chromosoma 101, 109–114 (1991). https://doi.org/10.1007/BF00357060

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  • DOI: https://doi.org/10.1007/BF00357060

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