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Osteosarcomagenic doses of radium (224Ra) and infectious endogenous retroviruses enhance proliferation and osteogenic differentiation of skeletal tissue differentiating in vitro

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Abstract

Cartilage tissue from embryonic mice which undergoes osteogenic differentiation during in vitro cultivation was used to study the effect of osteosarcomagenic doses of α-irradiation and bone-tumor-inducing retroviruses on proliferation and phenotypic differentiation of skeletal cells in a defined tissue culture model. Irradiated mandibular condyles showed dose-dependent enhancement of cell proliferation at day 7 of the culture and increased osteogenic differentiation at day 14. Maximal effects were found with 7.4 Bq/ml of224Ra-labeled medium. Doses of 740 and 7400 Bq/ml of224Ra-labeled medium induced increasing cell death. Retrovirus infection enhanced osteogenic differentiation and extended the viability of irradiated cells. After transplantation none of the treated tissues developed tumors in syngeneic mice.

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Authors and Affiliations

  1. Institut für Molekulare Virologie, GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Neuherberg, D-85758, Oberschleißheim, Germany

    Jörg Schmidt, Kathrin Heermeier & Volker Erfle

  2. Institut für Pathologie, GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Neuherberg, D-85758, Oberschleißheim, Germany

    Utz Linzner & Arne Luz

  3. Laboratory of Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, The Bruce Rappaport Faculty of Medicine, Technion, 31096, Haifa, Israel

    Michael Silbermann & Erella Livne

Authors
  1. Jörg Schmidt
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  2. Kathrin Heermeier
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  3. Utz Linzner
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  4. Arne Luz
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  5. Michael Silbermann
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  6. Erella Livne
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  7. Volker Erfle
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Schmidt, J., Heermeier, K., Linzner, U. et al. Osteosarcomagenic doses of radium (224Ra) and infectious endogenous retroviruses enhance proliferation and osteogenic differentiation of skeletal tissue differentiating in vitro. Radiat Environ Biophys 33, 69–79 (1994). https://doi.org/10.1007/BF01255275

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  • DOI: https://doi.org/10.1007/BF01255275

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