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Changes of carp myosin subfragment-1 induced by temperature acclimation

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Summary

Heavy meromyosin subfragment-1 (S1) was prepared by α-chymotrypsin from myosin of carp acclimated to either 10°C or 30°C for a minimum of 5 weeks. The objective of these studies was to document thermally-induced changes in the myosin molecule and to extend previous observations. Ca2+- and K+ (EDTA)-ATPase activities of cold-acclimated carp S1 were 1.1 and 0.8 μmol Pi·min-1·mg-1, respectively, and these values did not differ significantly from those of warm-acclimated carp. The inactivation rate constant (KD) of S1 from cold-acclimated carp was 32.1x10-4· s-1, compared to 13.2x10-4·s-1 for warm-acclimated carp. The maximum initial velocity of acto-S1 Mg2+-ATPase activity at pH 7.0 in 0.05 M KCl was 9.3 s-1 with cold-acclimated carp, about 3.7 times higher than that for warm-acclimated carp. However, no significant difference was observed in the apparent affinity of S1 to actin. Peptides maps of the heavy chain of S1 were different and suggested distinct isoforms for the myosins from warm- and cold-acclimated muscle.

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Abbreviations

ATPase :

adenosine 5′-triphosphatase

DTNB :

5,5′-dithiobis (2-nitrobenzoic acid)

DTT :

dithiothreitol

EDTA :

ethylenediaminetetraacetic acid

EGTA :

ethyleneglycol bis (β-aminoethylether)-N,N,N′,N′-tetraacetic acid

K D :

inactivation rate constant

K m :

apparent dissociation constant

P i :

inorganic γ-phosphate

PMSF :

phenylmethane-sulfonyl fluoride

S 1 :

heavy meromyosin subfragment-1

SDS :

sodium dodecyl sulfate

SDS-PAGE :

SDS-polyacrylamide gel electrophoresis

TPCK :

N-tosyl-l-phenylalanyl chloromethyl ketone

V max :

maximum initial velocity

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Authors and Affiliations

  1. Laboratory of Marine Biochemistry, Faculty of Agriculture, The University of Tokyo, 113, Bunkyo, Tokyo, Japan

    Gyu-Chul Hwang, Yoshihiro Ochiai & Shugo Watabe

  2. Food Science Laboratory, Faculty of Education, Ibaraki University, 310, Mito, Ibaraki, Japan

    Kanehisa Hashimoto

Authors
  1. Gyu-Chul Hwang
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  2. Yoshihiro Ochiai
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  3. Shugo Watabe
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  4. Kanehisa Hashimoto
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Hwang, GC., Ochiai, Y., Watabe, S. et al. Changes of carp myosin subfragment-1 induced by temperature acclimation. J Comp Physiol B 161, 141–146 (1991). https://doi.org/10.1007/BF00262876

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  • DOI: https://doi.org/10.1007/BF00262876

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