Summary
Fluorescence intensity of isolated salivary gland cells of wild-type and arrhythmic (pero)Drosophila melanogaster larvae was measured in constant conditions. The glands were incubated in a medium containing 3.5×10−6M 3,3′-dihexyl-oxacarbocyanine iodide for which cellular concentrations is probably controlled by the membrane potential. In wild-type cells significant rhythmicity of fluorescence intensity was measured over the cytoplasmic as well as the nuclear areas. In pero cells, arrhythmic and rhythmic changes were registered, the latter showing lower amplitudes. We conclude that the decreased amplitude and the lower number of significant cellular rhythms in pero mutants might lead to an apparent arrhythmicity at the behavioural level, i.e. activity and eclosion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antonov VF, Kurella G, Meshchishen J, Ben Tsze (1969) The action of potassium, sodium and chloride ions on the potential difference between medium, cytoplasm and the nucleus of salivary gland cells ofDrosophila larvae. Dokl Akad Nauk SSSR 161:691–693
Behnel HJ, Seydewitz HH (1980) Changes of membrane potential during the induction of heat shock puffs inDrosophila larval salivary glands. Exp Cell Res 127:133–141
Brady J (1974) The physiology of insect circadian rhythms. Adv Insect Physiol 10:1–115
Burckhardt G (1977) Non-linear relationship between fluorescence andmembrane potential. Biochim Biophys Acta 468:227–237
Cohen LB, Salzberg BM, Davila HV, Ross WN (1974) Changes in axon fluorescence during activity: Molecular probes of membrane potential. J Membr Biol 19:1–36
Drescher K, Timm J, Rensing L, Graf G, Cornelius G (1980) Statistical evaluation of biological rhythms in short, non-synchronous time series. J Interdiscip Cycle Res 11:69–83
Engelmann W, Schrempf M (1980) Membrane models for circadian rhythms. Photochem Photobiol Rev 5:49–87
Eskin A (1971) Properties of theAplysia visual system: in vitro entrainment of the circadian rhythm of centrifugal regulation of the eye. Z Vergl Physiol 74:353–371
Eskin A (1979) Identification and physiology of circadian pacemakers. Fed Proc 38:2570–2572
Handler AM, Konopka RJ (1979) Transplantation of a circadian pacemaker inKrosophila. Nature 279:236
Hardeland R (1973) Circadian rhythmicity in cultured liver cells. I. Rhythms in tyrosine aminotransferase activity and inducibility and in3H-leucine incorporation. Int J Biochem 4:581–590
Hoffman JF, Laris PD (1974) Determination of membrane potentials in human andAmphiuma red blood cells by means of a fluorescent probe. J Physiol 239:591–552
Jacklet JW (1977) Neuronal circadian rhythm: Phase shifting by a protein synthesis inhibitor. Science 198:69–71
Karakashian MW, Schweiger H-G (1976) Evidence for a cycloheximide-sensitive component in the biological clock ofAcetabularia. Exp Cell Res 98:303–321
Kinnally KW, Tedeschi H (1978) Metabolic effects of some electrofluorimetric dyes. Biochim Biophys Acta 503:380–388
Konopka RJ (1979) Genetic dissection of theDrosophila circadian system. Fed Proc 38:2602–2605
Konopka RJ, Benzer S (1971) Clock mutants ofDrosophila melanogaster. Proc Natl Acad Sci USA 68:2112–2116
Loewenstein WR, Kanno Y (1963) Some electrical properties of a nuclear membrane examined with a microelectrode. J Gen Physiol 46:1123–1140
Lukat R (1978) Circadian growth layers in the cuticle of behaviourally arhythmic cockroaches (Blaberus fucus, Ins. Blattodea). Experientia 34:477
Moore-Ede MC, Sulzman FM (1977) The physiological basis of circadian timekeeping in primates. Physiologist 20:17–25
Nagel G, Rensing L (1974) Circadian rhythm in size and3H-uridine incorporation of single puffs ofDrosophila salivary glands in vitro. Exp Cell Res 89:436–439
Njus D, Sulzmann FM, Hastings JW (1974) Membrane model for the circadian clock. Nature 248:116–120
Rensing L (1966) Zur circadianen Rhythmik des Sauerstoffverbrauches vonDrosophila. Z Vergl Physiol 53:62–83
Rensing L (1969) Circadiane Rhythmik vonDrosophila Speicheldrüsen in vivo, in vitro und nach Ecdyson-Zugabe. J Insect Physiol 15:2285–2303
Rensing L (1971) Hormonal control of circadian rhythms inDrosophila. In: Menaker M (ed) Biochronometry. Natl Acad Sci, Washington, DC, pp 527–540
Rensing L (1972) Periodic geophysical and biological signals as Zeitgeber and exogenous inducers in animal organisms. Biometeorology 5:113–125
Rensing L, Fischer M (1975) The effects of sodium, potassium and ATP on a developmental puff sequence inDrosophila salivary glands in vitro. Cell Diff 4:209–217
Rensing L, Hardeland R (1972) Effects of adenosine 3′,5′-cyclic monophosphate on membrane potential, nuclear volume and puff size ofDrosophila salivary glands in vitro. Exp Cell Res 73:311–318
Rensing L, Goedeke K, Wassmann G, Broich G (1974) Presence and absence of daily rhythms of nuclear size and DNA synthesis of different normal and transformed cells in culture. J Interdiscip Cycle Res 5:267–276
Rensing L, Behnel J, Marowsky G (1978) Differences of nuclear and cytoplasmic laser-induced fluorescence inDrosophila salivary gland cells. Cytobiologie 17:312–315
Rothman BS, Strumwasser F (1977) Manipulation of a neuronal circadian oscillator with inhibitors of macromolecular synthesis. Fed Proc 36:2050–2055
Sachs L (1974) Angewandte Statistik. Springer, Berlin Heidelberg New York
Schweiger E, Wallraf HG, Schweiger HG (1964) Endogenous circadian rhythm in the cytoplasm ofAcetabularia: influence of the nucleus. Science 146:658–659
Takahashi JS, Menaker M (1979) Physiology of avian circadian pacemakers. Fed Proc 38:2583–2588
Waggoner AS (1979) Dye indicators of membrane potential. Ann Rev Bioeng 8:47–68
Waggoner AS, Sirkin D, Tolles R, Wang CH (1975) Rate of membrane penetration of potential-sensitive dyes. Biophys J 15:20a
West W, Pearce S (1965) The dimeric state of cyanine dyes. J Phys Chem 69:1894–1903
Wever R (1965) A mathematical model for circadian rhythms. In: Aschoff J (ed) Circadian clocks. North-Holland, Amsterdam, pp 47–63
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weitzel, G., Rensing, L. Evidence for cellular circadian rhythms in isolated fluorescent dye-labelled salivary glands of wild type and an arrhythmic mutant ofDrosophila melanogaster . J Comp Physiol B 143, 229–235 (1981). https://doi.org/10.1007/BF00797702
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00797702